Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats
Objective:This study aims to observe the effects of transplantation of umbilical cord blood mononuclear cells (UCBMCs) on the expression of interleukin (IL)-1β and explore the mechanism via the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway in hypoxic-ischemic neonatal rats.Metho...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Journal of Neurorestoratology |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/JNR.2020.9040015 |
| _version_ | 1797757888507150336 |
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| author | Meng-Bei Zhang Chao-Chao Song Guang-Zu Li Lan-Fen Chen Rui Ma Xiao-He Yu Ping Gong Xiao-Li Wang |
| author_facet | Meng-Bei Zhang Chao-Chao Song Guang-Zu Li Lan-Fen Chen Rui Ma Xiao-He Yu Ping Gong Xiao-Li Wang |
| author_sort | Meng-Bei Zhang |
| collection | DOAJ |
| description | Objective:This study aims to observe the effects of transplantation of umbilical cord blood mononuclear cells (UCBMCs) on the expression of interleukin (IL)-1β and explore the mechanism via the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway in hypoxic-ischemic neonatal rats.Methods:Seven-day-old Sprague-Dawley neonatal rats were randomly divided into Sham, hypoxic-ischemic brain damage (HIBD), and UCBMC groups. The HIBD model was prepared by Rice-Vannucci method, and UCBMC were transplanted 24 h after HIBD in the UCBMC group. At 7 days after transplantation, changes in neurons and the TLR4 protein were examined by neuronal nuclei (NeuN)/TLR4 immunofluorescence staining. The expression of pNF-κB and IL-1β proteins was detected by immunohistochemical staining and enzyme linked immunosorbent assay (ELISA).Results:The percentage of NeuN+DAPI+ cells in the injured cortex in the UCBMC group was significantly higher than that in the HIBD group and lower than that in the Sham group (P +TLR4+DAPI+cells in the UCBMC group was significantly lower than that in the HIBD group (P P + cells were observed in the HIBD group than in Sham and UCBMC groups (P + cells were observed in the UCBMC group than in the Sham group (P P Conclusions:UCBMC transplantation could inhibit the IL-1β protein expression in the injured cortex, thereby alleviating HIBD in neonatal rats. The underlying mechanism might be associated with the down- regulation of TLR4 and pNF-κB proteins. |
| first_indexed | 2024-03-12T18:22:02Z |
| format | Article |
| id | doaj.art-5002c6421cad47ccbc072f81d14a1fb8 |
| institution | Directory Open Access Journal |
| issn | 2324-2426 |
| language | English |
| last_indexed | 2024-03-12T18:22:02Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Neurorestoratology |
| spelling | doaj.art-5002c6421cad47ccbc072f81d14a1fb82023-08-02T08:48:01ZengElsevierJournal of Neurorestoratology2324-24262020-06-018212213010.26599/JNR.2020.9040015Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal ratsMeng-Bei Zhang0Chao-Chao Song1Guang-Zu Li2Lan-Fen Chen3Rui Ma4Xiao-He Yu5Ping Gong6Xiao-Li Wang7<institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Digestive System</institution>, <institution>People’s Hospital of Fangzi District</institution>, <city>Weifang</city> <postal-code>261200</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Pediatrics, Xiangya Hospital</institution>, <institution>Central South University</institution>, <city>Changsha</city> <postal-code>410008</postal-code>, <state>Hunan</state>, <country>China</country><institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country><institution content-type="dept">Department of Medical Imaging</institution>, <institution>Weifang Medical University</institution>, <city>Weifang</city> <postal-code>261053</postal-code>, <state>Shandong</state>, <country>China</country>Objective:This study aims to observe the effects of transplantation of umbilical cord blood mononuclear cells (UCBMCs) on the expression of interleukin (IL)-1β and explore the mechanism via the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway in hypoxic-ischemic neonatal rats.Methods:Seven-day-old Sprague-Dawley neonatal rats were randomly divided into Sham, hypoxic-ischemic brain damage (HIBD), and UCBMC groups. The HIBD model was prepared by Rice-Vannucci method, and UCBMC were transplanted 24 h after HIBD in the UCBMC group. At 7 days after transplantation, changes in neurons and the TLR4 protein were examined by neuronal nuclei (NeuN)/TLR4 immunofluorescence staining. The expression of pNF-κB and IL-1β proteins was detected by immunohistochemical staining and enzyme linked immunosorbent assay (ELISA).Results:The percentage of NeuN+DAPI+ cells in the injured cortex in the UCBMC group was significantly higher than that in the HIBD group and lower than that in the Sham group (P +TLR4+DAPI+cells in the UCBMC group was significantly lower than that in the HIBD group (P P + cells were observed in the HIBD group than in Sham and UCBMC groups (P + cells were observed in the UCBMC group than in the Sham group (P P Conclusions:UCBMC transplantation could inhibit the IL-1β protein expression in the injured cortex, thereby alleviating HIBD in neonatal rats. The underlying mechanism might be associated with the down- regulation of TLR4 and pNF-κB proteins.https://www.sciopen.com/article/10.26599/JNR.2020.9040015umbilical cord blood mononuclear cell (ucbmc)hypoxic ischemic brain damage (hibd)toll-like receptor 4 (tlr4)nuclear factor-kappa b (nf-κb)tlr4/nf-κb pathwayneonatal rats |
| spellingShingle | Meng-Bei Zhang Chao-Chao Song Guang-Zu Li Lan-Fen Chen Rui Ma Xiao-He Yu Ping Gong Xiao-Li Wang Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats Journal of Neurorestoratology umbilical cord blood mononuclear cell (ucbmc) hypoxic ischemic brain damage (hibd) toll-like receptor 4 (tlr4) nuclear factor-kappa b (nf-κb) tlr4/nf-κb pathway neonatal rats |
| title | Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats |
| title_full | Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats |
| title_fullStr | Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats |
| title_full_unstemmed | Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats |
| title_short | Transplantation of umbilical cord blood mononuclear cells attenuates the expression of IL-1β via the TLR4/NF-κB pathway in hypoxic-ischemic neonatal rats |
| title_sort | transplantation of umbilical cord blood mononuclear cells attenuates the expression of il 1β via the tlr4 nf κb pathway in hypoxic ischemic neonatal rats |
| topic | umbilical cord blood mononuclear cell (ucbmc) hypoxic ischemic brain damage (hibd) toll-like receptor 4 (tlr4) nuclear factor-kappa b (nf-κb) tlr4/nf-κb pathway neonatal rats |
| url | https://www.sciopen.com/article/10.26599/JNR.2020.9040015 |
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