Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study
ObjectiveThere is some evidence for an association between gut microbiota and nonalcoholic fatty liver disease (NAFLD), alcoholic liver disease (ALD), and viral hepatitis, but no studies have explored their causal relationship.MethodsInstrumental variables of the gut microbiota (N = 13266) and gut m...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1159148/full |
| _version_ | 1797786697893675008 |
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| author | Lilong Zhang Lilong Zhang Lilong Zhang Liuliu Zi Liuliu Zi Liuliu Zi Tianrui Kuang Tianrui Kuang Tianrui Kuang Kunpeng Wang Kunpeng Wang Kunpeng Wang Zhendong Qiu Zhendong Qiu Zhendong Qiu Zhongkai Wu Zhongkai Wu Zhongkai Wu Li Liu Li Liu Li Liu Rongqiang Liu Rongqiang Liu Rongqiang Liu Peng Wang Peng Wang Peng Wang Weixing Wang Weixing Wang Weixing Wang |
| author_facet | Lilong Zhang Lilong Zhang Lilong Zhang Liuliu Zi Liuliu Zi Liuliu Zi Tianrui Kuang Tianrui Kuang Tianrui Kuang Kunpeng Wang Kunpeng Wang Kunpeng Wang Zhendong Qiu Zhendong Qiu Zhendong Qiu Zhongkai Wu Zhongkai Wu Zhongkai Wu Li Liu Li Liu Li Liu Rongqiang Liu Rongqiang Liu Rongqiang Liu Peng Wang Peng Wang Peng Wang Weixing Wang Weixing Wang Weixing Wang |
| author_sort | Lilong Zhang |
| collection | DOAJ |
| description | ObjectiveThere is some evidence for an association between gut microbiota and nonalcoholic fatty liver disease (NAFLD), alcoholic liver disease (ALD), and viral hepatitis, but no studies have explored their causal relationship.MethodsInstrumental variables of the gut microbiota (N = 13266) and gut microbiota-derived metabolites (N = 7824) were acquired, and a Mendelian randomization study was performed to explore their influence on NAFLD (1483 European cases and 17,781 European controls), ALD (2513 European cases and 332,951 European controls), and viral hepatitis risk (1971 European cases and 340,528 European controls). The main method for examining causality is inverse variance weighting (IVW).ResultsIVW results confirmed that Anaerotruncus (p = 0.0249), Intestinimonas (p = 0.0237), Lachnoclostridium (p = 0.0245), Lachnospiraceae NC2004 group (p = 0.0083), Olsenella (p = 0.0163), and Peptococcus (p = 0.0472) were protective factors for NAFLD, and Ruminococcus 1 (p = 0.0120) was detrimental for NAFLD. The higher abundance of three genera, Lachnospira (p = 0.0388), Desulfovibrio (p = 0.0252), and Ruminococcus torques group (p = 0.0364), was correlated with a lower risk of ALD, while Ruminococcaceae UCG 002 level was associated with a higher risk of ALD (p = 0.0371). The Alistipes (p = 0.0069) and Ruminococcaceae NK4A214 group (p = 0.0195) were related to a higher risk of viral hepatitis. Besides, alanine (p = 0.0076) and phenyllactate (p = 0.0100) were found to be negatively correlated with NAFLD, while stachydrine (Op = 0.0244) was found to be positively associated with NAFLD. The phenylacetate (p = 0.0353) and ursodeoxycholate (p = 0.0144) had a protective effect on ALD, while the threonate (p = 0.0370) exerted a detrimental influence on ALD. The IVW estimates of alanine (p = 0.0408) and cholate (p = 0.0293) showed their suggestive harmful effects against viral hepatitis, while threonate (p = 0.0401) displayed its suggestive protective effect against viral hepatitis.ConclusionIn conclusion, our research supported causal links between the gut microbiome and its metabolites and NAFLD, ALD, and viral hepatitis. |
| first_indexed | 2024-03-13T01:11:36Z |
| format | Article |
| id | doaj.art-500bfb7e0aac47668bd479a7f8c0c677 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-13T01:11:36Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-500bfb7e0aac47668bd479a7f8c0c6772023-07-05T17:51:40ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-07-011410.3389/fendo.2023.11591481159148Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization studyLilong Zhang0Lilong Zhang1Lilong Zhang2Liuliu Zi3Liuliu Zi4Liuliu Zi5Tianrui Kuang6Tianrui Kuang7Tianrui Kuang8Kunpeng Wang9Kunpeng Wang10Kunpeng Wang11Zhendong Qiu12Zhendong Qiu13Zhendong Qiu14Zhongkai Wu15Zhongkai Wu16Zhongkai Wu17Li Liu18Li Liu19Li Liu20Rongqiang Liu21Rongqiang Liu22Rongqiang Liu23Peng Wang24Peng Wang25Peng Wang26Weixing Wang27Weixing Wang28Weixing Wang29Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaHubei Key Laboratory of Digestive System Disease, Wuhan, Hubei, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, ChinaObjectiveThere is some evidence for an association between gut microbiota and nonalcoholic fatty liver disease (NAFLD), alcoholic liver disease (ALD), and viral hepatitis, but no studies have explored their causal relationship.MethodsInstrumental variables of the gut microbiota (N = 13266) and gut microbiota-derived metabolites (N = 7824) were acquired, and a Mendelian randomization study was performed to explore their influence on NAFLD (1483 European cases and 17,781 European controls), ALD (2513 European cases and 332,951 European controls), and viral hepatitis risk (1971 European cases and 340,528 European controls). The main method for examining causality is inverse variance weighting (IVW).ResultsIVW results confirmed that Anaerotruncus (p = 0.0249), Intestinimonas (p = 0.0237), Lachnoclostridium (p = 0.0245), Lachnospiraceae NC2004 group (p = 0.0083), Olsenella (p = 0.0163), and Peptococcus (p = 0.0472) were protective factors for NAFLD, and Ruminococcus 1 (p = 0.0120) was detrimental for NAFLD. The higher abundance of three genera, Lachnospira (p = 0.0388), Desulfovibrio (p = 0.0252), and Ruminococcus torques group (p = 0.0364), was correlated with a lower risk of ALD, while Ruminococcaceae UCG 002 level was associated with a higher risk of ALD (p = 0.0371). The Alistipes (p = 0.0069) and Ruminococcaceae NK4A214 group (p = 0.0195) were related to a higher risk of viral hepatitis. Besides, alanine (p = 0.0076) and phenyllactate (p = 0.0100) were found to be negatively correlated with NAFLD, while stachydrine (Op = 0.0244) was found to be positively associated with NAFLD. The phenylacetate (p = 0.0353) and ursodeoxycholate (p = 0.0144) had a protective effect on ALD, while the threonate (p = 0.0370) exerted a detrimental influence on ALD. The IVW estimates of alanine (p = 0.0408) and cholate (p = 0.0293) showed their suggestive harmful effects against viral hepatitis, while threonate (p = 0.0401) displayed its suggestive protective effect against viral hepatitis.ConclusionIn conclusion, our research supported causal links between the gut microbiome and its metabolites and NAFLD, ALD, and viral hepatitis.https://www.frontiersin.org/articles/10.3389/fendo.2023.1159148/fullalcoholic liver diseasenonalcoholic fatty liver diseaseviral hepatitisgut microbiotagut microbiota-derived metabolitesmendelian randomization analysis |
| spellingShingle | Lilong Zhang Lilong Zhang Lilong Zhang Liuliu Zi Liuliu Zi Liuliu Zi Tianrui Kuang Tianrui Kuang Tianrui Kuang Kunpeng Wang Kunpeng Wang Kunpeng Wang Zhendong Qiu Zhendong Qiu Zhendong Qiu Zhongkai Wu Zhongkai Wu Zhongkai Wu Li Liu Li Liu Li Liu Rongqiang Liu Rongqiang Liu Rongqiang Liu Peng Wang Peng Wang Peng Wang Weixing Wang Weixing Wang Weixing Wang Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study Frontiers in Endocrinology alcoholic liver disease nonalcoholic fatty liver disease viral hepatitis gut microbiota gut microbiota-derived metabolites mendelian randomization analysis |
| title | Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study |
| title_full | Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study |
| title_fullStr | Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study |
| title_full_unstemmed | Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study |
| title_short | Investigating causal associations among gut microbiota, metabolites, and liver diseases: a Mendelian randomization study |
| title_sort | investigating causal associations among gut microbiota metabolites and liver diseases a mendelian randomization study |
| topic | alcoholic liver disease nonalcoholic fatty liver disease viral hepatitis gut microbiota gut microbiota-derived metabolites mendelian randomization analysis |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1159148/full |
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