Intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells
Summary: Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27− γδ T cells, and ATP delivery. We then...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004999 |