Intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells

Intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells

Summary: Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27− γδ T cells, and ATP delivery. We then...

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Bibliographic Details
Main Authors: Yunke Wang, Jun Yan
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Culture
Cell Isolation
Flow Cytometry/Mass Cytometry
Health Sciences
Immunology
Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004999
Description
Summary:Summary: Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27− γδ T cells, and ATP delivery. We then detail functional evaluation of γδ T cells by flow cytometry. Appropriate concentrations of control and ATP vesicles are detailed for intracellular ATP delivery, which can also be applied to other immune cells.For complete details on the use and execution of this protocol, please refer to Wang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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