An optimized protocol for isolation of hepatic leukocytes retrieved from murine and NASH liver biopsies

An optimized protocol for isolation of hepatic leukocytes retrieved from murine and NASH liver biopsies

Summary: Immune dysregulation and inflammation by hepatic-resident leukocytes is considered a key step in disease progression of non-alcoholic fatty liver disease and non-alcoholic steatohepatitis toward cirrhosis and hepatocellular carcinoma. Here, we provide a protocol for isolation and characteri...

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Main Authors: Ehsan Bahrami, Tobias Geiger, Agnes A. Steixner-Kumar, Diana Santacruz, Coralie Viollet, Alec Dick, Yvonne Roth, Patrycja Schlingeloff, Julian Schmidberger, Mark Haenle, Wolfgang Kratzer, Kerstin Kitt, Heike Neubauer, Eric Simon, Oliver Krenkel, Markus Werner
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Isolation
Flow Cytometry/Mass Cytometry
Health Sciences
Clinical Protocol
Sequencing
RNA-seq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005646

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http://www.sciencedirect.com/science/article/pii/S2666166723005646

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