Development and Validation of a Novel COVID-19 nsp8 One-Tube RT-LAMP-CRISPR Assay for SARS-CoV-2 Diagnosis

Development and Validation of a Novel COVID-19 nsp8 One-Tube RT-LAMP-CRISPR Assay for SARS-CoV-2 Diagnosis

ABSTRACT Accurate and simple diagnostic tests for coronavirus disease 2019 (COVID-19) are essential components of the pandemic response. In this study, we evaluated a one-tube reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay coupled with clustered regularly interspaced sh...

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Bibliographic Details
Main Authors: Cyril Chik-Yan Yip, Siddharth Sridhar, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Kit-Hang Leung, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To
Format: Article
Language:English
Published: American Society for Microbiology 2022-12-01
Series:Microbiology Spectrum
Subjects:
SARS-CoV-2
nsp8
RT-LAMP
CRISPR
diagnostic assay
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01962-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.01962-22

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