A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding

A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding

This study examines the potential of next-generation sequencing based ‘genotyping-by-sequencing’ (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorpor...

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Bibliographic Details
Main Authors: Salla Vartia, José L. Villanueva-Cañas, John Finarelli, Edward D. Farrell, Patrick C. Collins, Graham M. Hughes, Jeanette E. L. Carlsson, David T. Gauthier, Philip McGinnity, Thomas F. Cross, Richard D. FitzGerald, Luca Mirimin, Fiona Crispie, Paul D. Cotter, Jens Carlsson
Format: Article
Language:English
Published: The Royal Society 2016-01-01
Series:Royal Society Open Science
Subjects:
amplicon sequencing
gadus morhua
genotyping by sequencing
next-generation sequencing
ssr
universal primer
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150565

Internet

https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.150565

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