Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection
Several plant extracts exhibit anti-virulence properties due to the interruption of bacterial quorum sensing (QS). However, studies on their effects at the preclinical level are scarce. Here, we used a murine model of abscess/necrosis induced by <i>Pseudomonas aeruginosa</i> to evaluate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/12/2424 |
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| author | José Luis Díaz-Núñez Macrina Pérez-López Norma Espinosa Nayelli Campos-Hernández Rodolfo García-Contreras Miguel Díaz-Guerrero Humberto Cortes-López Monserrat Vázquez-Sánchez Héctor Quezada Mariano Martínez-Vázquez Ramón Marcos Soto-Hernández Mireya Burgos-Hernández Bertha González-Pedrajo Israel Castillo-Juárez |
| author_facet | José Luis Díaz-Núñez Macrina Pérez-López Norma Espinosa Nayelli Campos-Hernández Rodolfo García-Contreras Miguel Díaz-Guerrero Humberto Cortes-López Monserrat Vázquez-Sánchez Héctor Quezada Mariano Martínez-Vázquez Ramón Marcos Soto-Hernández Mireya Burgos-Hernández Bertha González-Pedrajo Israel Castillo-Juárez |
| author_sort | José Luis Díaz-Núñez |
| collection | DOAJ |
| description | Several plant extracts exhibit anti-virulence properties due to the interruption of bacterial quorum sensing (QS). However, studies on their effects at the preclinical level are scarce. Here, we used a murine model of abscess/necrosis induced by <i>Pseudomonas aeruginosa</i> to evaluate the anti-pathogenic efficacy of 24 plant extracts at a sub-inhibitory concentration. We analyzed their ability to inhibit QS-regulated virulence factors such as swarming, pyocyanin production, and secretion of the ExoU toxin via the type III secretion system (T3SS). Five of the seven extracts with the best anti-pathogenic activity reduced ExoU secretion, and the extracts of <i>Diphysa americana</i> and <i>Hibiscus sabdariffa</i> were identified as the most active. Therefore, the abscess/necrosis model allows identification of plant extracts that have the capacity to reduce pathogenicity of <i>P. aeruginosa</i>. Furthermore, we evaluated the activity of the plant extracts on <i>Chromobacterium violaceum</i>. T3SS (Δ<i>escU</i>) and QS (Δ<i>cviI</i>) mutant strains were assessed in both the abscess/necrosis and sepsis models. Only the Δ<i>escU</i> strain had lower pathogenicity in the animal models, although no activity of plant extracts was observed. These results demonstrate differences between the anti-virulence activity recorded in vitro and pathogenicity in vivo and between the roles of QS and T3S systems as virulence determinants. |
| first_indexed | 2024-03-10T03:32:14Z |
| format | Article |
| id | doaj.art-50830f3377f047a48acd638f64678c6a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:32:14Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-50830f3377f047a48acd638f64678c6a2023-11-23T09:37:47ZengMDPI AGMicroorganisms2076-26072021-11-01912242410.3390/microorganisms9122424Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial InfectionJosé Luis Díaz-Núñez0Macrina Pérez-López1Norma Espinosa2Nayelli Campos-Hernández3Rodolfo García-Contreras4Miguel Díaz-Guerrero5Humberto Cortes-López6Monserrat Vázquez-Sánchez7Héctor Quezada8Mariano Martínez-Vázquez9Ramón Marcos Soto-Hernández10Mireya Burgos-Hernández11Bertha González-Pedrajo12Israel Castillo-Juárez13Laboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoLaboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartamento de Productos Naturales, Instituto de Química, Universidad Nacional Autόnoma de México, Ciudad de México 04510, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoLaboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, MexicoSeveral plant extracts exhibit anti-virulence properties due to the interruption of bacterial quorum sensing (QS). However, studies on their effects at the preclinical level are scarce. Here, we used a murine model of abscess/necrosis induced by <i>Pseudomonas aeruginosa</i> to evaluate the anti-pathogenic efficacy of 24 plant extracts at a sub-inhibitory concentration. We analyzed their ability to inhibit QS-regulated virulence factors such as swarming, pyocyanin production, and secretion of the ExoU toxin via the type III secretion system (T3SS). Five of the seven extracts with the best anti-pathogenic activity reduced ExoU secretion, and the extracts of <i>Diphysa americana</i> and <i>Hibiscus sabdariffa</i> were identified as the most active. Therefore, the abscess/necrosis model allows identification of plant extracts that have the capacity to reduce pathogenicity of <i>P. aeruginosa</i>. Furthermore, we evaluated the activity of the plant extracts on <i>Chromobacterium violaceum</i>. T3SS (Δ<i>escU</i>) and QS (Δ<i>cviI</i>) mutant strains were assessed in both the abscess/necrosis and sepsis models. Only the Δ<i>escU</i> strain had lower pathogenicity in the animal models, although no activity of plant extracts was observed. These results demonstrate differences between the anti-virulence activity recorded in vitro and pathogenicity in vivo and between the roles of QS and T3S systems as virulence determinants.https://www.mdpi.com/2076-2607/9/12/2424cutaneous infection modeltype III secretion systemquorum sensing<i>Chromobacterium violaceum</i><i>Pseudomonas aeruginosa</i><i>Diphysa americana</i> |
| spellingShingle | José Luis Díaz-Núñez Macrina Pérez-López Norma Espinosa Nayelli Campos-Hernández Rodolfo García-Contreras Miguel Díaz-Guerrero Humberto Cortes-López Monserrat Vázquez-Sánchez Héctor Quezada Mariano Martínez-Vázquez Ramón Marcos Soto-Hernández Mireya Burgos-Hernández Bertha González-Pedrajo Israel Castillo-Juárez Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection Microorganisms cutaneous infection model type III secretion system quorum sensing <i>Chromobacterium violaceum</i> <i>Pseudomonas aeruginosa</i> <i>Diphysa americana</i> |
| title | Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection |
| title_full | Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection |
| title_fullStr | Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection |
| title_full_unstemmed | Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection |
| title_short | Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection |
| title_sort | anti virulence properties of plant species correlation between in vitro activity and efficacy in a murine model of bacterial infection |
| topic | cutaneous infection model type III secretion system quorum sensing <i>Chromobacterium violaceum</i> <i>Pseudomonas aeruginosa</i> <i>Diphysa americana</i> |
| url | https://www.mdpi.com/2076-2607/9/12/2424 |
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