Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows
Abstract This experiment was conducted to evaluate the impact of yeast and lactic acid bacteria (LAB) on mastitis and milk microbiota composition of dairy cows. Thirty lactating Holstein cows with similar parity, days in milk were randomly assigned to five treatments, including: (1) Health cows with...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-01-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-020-0953-8 |
| _version_ | 1818412670536122368 |
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| author | Jing Gao Yu-Chen Liu Yu Wang Han Li Xiang-Ming Wang Yan Wu Ding-Ran Zhang Si Gao Zhi-li Qi |
| author_facet | Jing Gao Yu-Chen Liu Yu Wang Han Li Xiang-Ming Wang Yan Wu Ding-Ran Zhang Si Gao Zhi-li Qi |
| author_sort | Jing Gao |
| collection | DOAJ |
| description | Abstract This experiment was conducted to evaluate the impact of yeast and lactic acid bacteria (LAB) on mastitis and milk microbiota composition of dairy cows. Thirty lactating Holstein cows with similar parity, days in milk were randomly assigned to five treatments, including: (1) Health cows with milk SCC < 500,000 cells/mL, no clinical signs of mastitis were found, fed basal total mixed ration (TMR) without supplementation (H); (2) Mastitis cows with milk SCC > 500,000 cells/mL, fed basal TMR without supplementation (M); (3) Mastitis cows fed basal TMR supplemented with 8 g day−1 yeast (M + Y); (4) Mastitis cows fed basal TMR supplemented with 8 g day−1 LAB (M + L); (5) Mastitis cows (milk SCC > 500,000 cells/mL) fed basal TMR supplemented with 4 g day−1 yeast and 4 g day−1 LAB (M + Y + L). Blood and milk sample were collected at day 0, day 20 and day 40. The results showed efficacy of probiotic: On day 20 and day 40, milk SCC in H, M + Y, M + L, M + Y + L was significantly lower than that of M (P < 0.05). Milk concentration of TNF-α, IL-6 and IL-1β in M + Y + L were significantly reduced compared with that of M on day 40 (P < 0.05). Milk Myeloperoxidase (MPO) and N-Acetyl-β-d-Glucosaminidase (NAG) activity of M + Y, M + L, M + L + Y were lower than that of M on day 40 (P < 0.05). At genus level, Staphylococcus, Chryseobacterium and Lactococcus were dominant. Supplementation of LAB decreased abundance of Enterococcus and Streptococcus, identified as mastitis-causing pathogen. The results suggested the potential of LAB to prevent mastitis by relieving mammary gland inflammation and regulating milk microorganisms. |
| first_indexed | 2024-12-14T10:51:00Z |
| format | Article |
| id | doaj.art-50a918370de34c05a295df1478fef854 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T10:51:00Z |
| publishDate | 2020-01-01 |
| publisher | SpringerOpen |
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| series | AMB Express |
| spelling | doaj.art-50a918370de34c05a295df1478fef8542022-12-21T23:05:14ZengSpringerOpenAMB Express2191-08552020-01-0110111210.1186/s13568-020-0953-8Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cowsJing Gao0Yu-Chen Liu1Yu Wang2Han Li3Xiang-Ming Wang4Yan Wu5Ding-Ran Zhang6Si Gao7Zhi-li Qi8Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural UniversityAbstract This experiment was conducted to evaluate the impact of yeast and lactic acid bacteria (LAB) on mastitis and milk microbiota composition of dairy cows. Thirty lactating Holstein cows with similar parity, days in milk were randomly assigned to five treatments, including: (1) Health cows with milk SCC < 500,000 cells/mL, no clinical signs of mastitis were found, fed basal total mixed ration (TMR) without supplementation (H); (2) Mastitis cows with milk SCC > 500,000 cells/mL, fed basal TMR without supplementation (M); (3) Mastitis cows fed basal TMR supplemented with 8 g day−1 yeast (M + Y); (4) Mastitis cows fed basal TMR supplemented with 8 g day−1 LAB (M + L); (5) Mastitis cows (milk SCC > 500,000 cells/mL) fed basal TMR supplemented with 4 g day−1 yeast and 4 g day−1 LAB (M + Y + L). Blood and milk sample were collected at day 0, day 20 and day 40. The results showed efficacy of probiotic: On day 20 and day 40, milk SCC in H, M + Y, M + L, M + Y + L was significantly lower than that of M (P < 0.05). Milk concentration of TNF-α, IL-6 and IL-1β in M + Y + L were significantly reduced compared with that of M on day 40 (P < 0.05). Milk Myeloperoxidase (MPO) and N-Acetyl-β-d-Glucosaminidase (NAG) activity of M + Y, M + L, M + L + Y were lower than that of M on day 40 (P < 0.05). At genus level, Staphylococcus, Chryseobacterium and Lactococcus were dominant. Supplementation of LAB decreased abundance of Enterococcus and Streptococcus, identified as mastitis-causing pathogen. The results suggested the potential of LAB to prevent mastitis by relieving mammary gland inflammation and regulating milk microorganisms.https://doi.org/10.1186/s13568-020-0953-8Lactic acid bacteriaYeastMastitisMilk microbiota compositionDairy cows |
| spellingShingle | Jing Gao Yu-Chen Liu Yu Wang Han Li Xiang-Ming Wang Yan Wu Ding-Ran Zhang Si Gao Zhi-li Qi Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows AMB Express Lactic acid bacteria Yeast Mastitis Milk microbiota composition Dairy cows |
| title | Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| title_full | Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| title_fullStr | Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| title_full_unstemmed | Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| title_short | Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| title_sort | impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows |
| topic | Lactic acid bacteria Yeast Mastitis Milk microbiota composition Dairy cows |
| url | https://doi.org/10.1186/s13568-020-0953-8 |
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