A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean
The faba bean is one of the earliest domesticated crops, with both economic and environmental benefits. Like most legumes, faba beans are high in protein, and can be used to contribute to a balanced diet, or as a meat substitute. However, they also produce the anti-nutritional compounds, vicine and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6288 |
| _version_ | 1827653857437548544 |
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| author | Aaron C. Elkins Simone J. Rochfort Pankaj Maharjan Joe Panozzo |
| author_facet | Aaron C. Elkins Simone J. Rochfort Pankaj Maharjan Joe Panozzo |
| author_sort | Aaron C. Elkins |
| collection | DOAJ |
| description | The faba bean is one of the earliest domesticated crops, with both economic and environmental benefits. Like most legumes, faba beans are high in protein, and can be used to contribute to a balanced diet, or as a meat substitute. However, they also produce the anti-nutritional compounds, vicine and convicine (v-c), that when enzymatically degraded into reactive aglycones can potentially lead to hemolytic anemia or favism. Current methods of analysis use LC-UV, but are only suitable at high concentrations, and thus lack the selectivity and sensitivity to accurately quantitate the low-v-c genotypes currently being developed. We have developed and fully validated a rapid high-throughput LC-MS method for the analysis of v-c in faba beans by optimizing the extraction protocol and assessing the method of linearity, limit of detection, limit of quantitation, accuracy, precision and matrix effects. This method uses 10-times less starting material; removes the use of buffers, acids and organic chemicals; and improves precision and accuracy when compared to current methods. |
| first_indexed | 2024-03-09T21:25:35Z |
| format | Article |
| id | doaj.art-50b4676858004634aa7ae852728cc3dc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:25:35Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-50b4676858004634aa7ae852728cc3dc2023-11-23T21:08:52ZengMDPI AGMolecules1420-30492022-09-012719628810.3390/molecules27196288A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba BeanAaron C. Elkins0Simone J. Rochfort1Pankaj Maharjan2Joe Panozzo3Agriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaAgriculture Victoria, Grains Innovation Park, Horsham, VIC 3400, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, Bundoora, VIC 3083, AustraliaThe faba bean is one of the earliest domesticated crops, with both economic and environmental benefits. Like most legumes, faba beans are high in protein, and can be used to contribute to a balanced diet, or as a meat substitute. However, they also produce the anti-nutritional compounds, vicine and convicine (v-c), that when enzymatically degraded into reactive aglycones can potentially lead to hemolytic anemia or favism. Current methods of analysis use LC-UV, but are only suitable at high concentrations, and thus lack the selectivity and sensitivity to accurately quantitate the low-v-c genotypes currently being developed. We have developed and fully validated a rapid high-throughput LC-MS method for the analysis of v-c in faba beans by optimizing the extraction protocol and assessing the method of linearity, limit of detection, limit of quantitation, accuracy, precision and matrix effects. This method uses 10-times less starting material; removes the use of buffers, acids and organic chemicals; and improves precision and accuracy when compared to current methods.https://www.mdpi.com/1420-3049/27/19/6288vicineconvicinefaba beananti-nutritionalquantitationLC-MS |
| spellingShingle | Aaron C. Elkins Simone J. Rochfort Pankaj Maharjan Joe Panozzo A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean Molecules vicine convicine faba bean anti-nutritional quantitation LC-MS |
| title | A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean |
| title_full | A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean |
| title_fullStr | A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean |
| title_full_unstemmed | A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean |
| title_short | A Simple High-Throughput Method for the Analysis of Vicine and Convicine in Faba Bean |
| title_sort | simple high throughput method for the analysis of vicine and convicine in faba bean |
| topic | vicine convicine faba bean anti-nutritional quantitation LC-MS |
| url | https://www.mdpi.com/1420-3049/27/19/6288 |
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