Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases
Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2002-01-01
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| Series: | Journal of the Brazilian Chemical Society |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000200010 |
| _version_ | 1811242641592942592 |
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| author | Bonato Pierina S. Bortocan Renato Gaitani Cristiane M. Paias Fernanda O. Iha Maria H. Lima Rodrigo P. |
| author_facet | Bonato Pierina S. Bortocan Renato Gaitani Cristiane M. Paias Fernanda O. Iha Maria H. Lima Rodrigo P. |
| author_sort | Bonato Pierina S. |
| collection | DOAJ |
| description | Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under normal phase conditions, using hexane:2-propanol or hexane:ethanol as mobile phase. Diethylamine and trifluoracetic acid were also added to improve peak shape. The CHIRALCEL OJ-R, CHIRALCEL OD-R and CHIRALCEL OD-H columns were evaluated under reversed-phase conditions, using acetonitrile:H2O or acetonitrile:NaClO4 solution. The protein- based stationary phases, CHIRAL AGP and ULTRON ES-OVM columns were used with mobile phases consisting of a buffer solution supplemented with an organic modifier. Among the polysaccharide-based stationary phases, CHIRALPAK AD provided better resolution for almost all drugs and metabolites studied. The ULTRON ES-OVM column was particularly suitable for the resolution of the four enantiomers of thioridazine-2-sulfoxide. |
| first_indexed | 2024-04-12T13:54:02Z |
| format | Article |
| id | doaj.art-50bcb490cb734dde9694a04b136a414c |
| institution | Directory Open Access Journal |
| issn | 0103-5053 |
| language | English |
| last_indexed | 2024-04-12T13:54:02Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Journal of the Brazilian Chemical Society |
| spelling | doaj.art-50bcb490cb734dde9694a04b136a414c2022-12-22T03:30:25ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50532002-01-01132190199Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary PhasesBonato Pierina S.Bortocan RenatoGaitani Cristiane M.Paias Fernanda O.Iha Maria H.Lima Rodrigo P.Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under normal phase conditions, using hexane:2-propanol or hexane:ethanol as mobile phase. Diethylamine and trifluoracetic acid were also added to improve peak shape. The CHIRALCEL OJ-R, CHIRALCEL OD-R and CHIRALCEL OD-H columns were evaluated under reversed-phase conditions, using acetonitrile:H2O or acetonitrile:NaClO4 solution. The protein- based stationary phases, CHIRAL AGP and ULTRON ES-OVM columns were used with mobile phases consisting of a buffer solution supplemented with an organic modifier. Among the polysaccharide-based stationary phases, CHIRALPAK AD provided better resolution for almost all drugs and metabolites studied. The ULTRON ES-OVM column was particularly suitable for the resolution of the four enantiomers of thioridazine-2-sulfoxide.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000200010polysaccharide-based stationary phasesprotein- based stationary phasesdrugsmetabolitesenantioselective analysis |
| spellingShingle | Bonato Pierina S. Bortocan Renato Gaitani Cristiane M. Paias Fernanda O. Iha Maria H. Lima Rodrigo P. Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases Journal of the Brazilian Chemical Society polysaccharide-based stationary phases protein- based stationary phases drugs metabolites enantioselective analysis |
| title | Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases |
| title_full | Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases |
| title_fullStr | Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases |
| title_full_unstemmed | Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases |
| title_short | Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases |
| title_sort | enantiomeric resolution of drugs and metabolites in polysaccharide and protein based chiral stationary phases |
| topic | polysaccharide-based stationary phases protein- based stationary phases drugs metabolites enantioselective analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000200010 |
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