Next-generation sequencing of a combinatorial peptide phage library screened against ubiquitin identifies peptide aptamers that can inhibit the in vitro ubiquitin transfer cascade

Next-generation sequencing of a combinatorial peptide phage library screened against ubiquitin identifies peptide aptamers that can inhibit the in vitro ubiquitin transfer cascade

Defining dynamic protein–protein interactions in the ubiquitin conjugation reaction is a challenging research area. Generating peptide aptamers that target components such as ubiquitin itself, E1, E2, or E3 could provide tools to dissect novel features of the enzymatic cascade. Next-generation deep...

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Bibliographic Details
Main Authors: Małgorzata Lisowska, Fiona Lickiss, Maria Gil-Mir, Anne-Sophie Huart, Zuzanna Trybala, Luke Way, Lenka Hernychova, Adam Krejci, Petr Muller, Radovan Krejcir, Igor Zhukow, Przemyslaw Jurczak, Sylwia Rodziewicz-Motowidło, Kathryn Ball, Borivoj Vojtesek, Ted Hupp, Umesh Kalathiya
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Microbiology
Subjects:
ubiquitin
phage-peptide
next-generation sequencing
aptamers
molecular dynamics
protein–peptide binding
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.875556/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.875556/full

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