Two-color fluorescence detection of Poly (ADP-Ribose) Polymerase-1 (PARP-1) cleavage and DNA strand breaks in etoposide-induced apoptotic cells

Two-color fluorescence detection of Poly (ADP-Ribose) Polymerase-1 (PARP-1) cleavage and DNA strand breaks in etoposide-induced apoptotic cells

During apoptosis, the nuclear enzyme Poly(ADPRibose) Polymerase-1 (PARP-1) catalyzes the rapid and transient synthesis of poly(ADP-ribose) from NAD+ and becomes inactive when cleaved by caspases. The regulation of these two opposite roles of PARP-1 is still unknown. We have recently investigated PAR...

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Bibliographic Details
Main Authors: C Soldani, MG Bottone, C Pellicciari, AI Scovassi
Format: Article
Language:English
Published: PAGEPress Publications 2009-12-01
Series:European Journal of Histochemistry
Subjects:
apoptosis
etoposide
fluorescence
immunocytochemistry
PARP-1 cleavage
TUNEL.
Online Access:https://www.ejh.it/index.php/ejh/article/view/1648

Internet

https://www.ejh.it/index.php/ejh/article/view/1648

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