Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry

This protocol describes a straightforward technique to evaluate the phagocytotic capacity of murine macrophages for Staphylococcus aureus (S. aureus). By staining S. aureus with Hexidium Iodide and staining murine bone marrow-derived macrophages (BMDMs) with FITC, the macrophage bacterial up-taking...

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Bibliographic Details
Main Authors: Qin Yan, Sun Ahn, Vance Fowler
Format: Article
Language:English
Published: Bio-protocol LLC 2015-02-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1406&type=0