Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway
This study aimed to assess the effects of triterpene extract of Cyclocarya paliurus (Batal.) Iljinskaja (CPT) on diabetes-induced hepatic inflammation and to unveil the underlying mechanisms. Diabetes in db/db mice was alleviated after CPT administration, as assessed by the oral glucose tolerance te...
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| Format: | Article |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00811/full |
| _version_ | 1818144157205528576 |
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| author | Cuihua Jiang Cuihua Jiang Cuihua Jiang Yiting Wang Qiaomei Jin Qiaomei Jin Dongjian Zhang Dongjian Zhang Meng Gao Meng Gao Nan Yao Nan Yao Zhiqi Yin Jian Zhang Jian Zhang Shiping Ma |
| author_facet | Cuihua Jiang Cuihua Jiang Cuihua Jiang Yiting Wang Qiaomei Jin Qiaomei Jin Dongjian Zhang Dongjian Zhang Meng Gao Meng Gao Nan Yao Nan Yao Zhiqi Yin Jian Zhang Jian Zhang Shiping Ma |
| author_sort | Cuihua Jiang |
| collection | DOAJ |
| description | This study aimed to assess the effects of triterpene extract of Cyclocarya paliurus (Batal.) Iljinskaja (CPT) on diabetes-induced hepatic inflammation and to unveil the underlying mechanisms. Diabetes in db/db mice was alleviated after CPT administration, as assessed by the oral glucose tolerance test. In addition, treatment with CPT dramatically reduced serum insulin, aspartate amino-transaminase, alanine aminotransferase, triglyceride, and total cholesterol amounts. Besides, serum levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were also reduced after CPT administration. Western blot analysis revealed that CPT treatment significantly reversed the protein expression levels of Rho, ROCK1, ROCK2, p-P65, p-IκBα, p-IKKα, and p-IKKβ in liver samples obtained from db/db mice. Upon palmitic acid stimulation, the protective effects of CPT on the liver were further assessed in HepG2 and LO2 cells, and no appreciable cytotoxic effects were found. Therefore, these findings indicate that CPT alleviates liver inflammation via Rho-kinase signaling.Chemical compounds evaluated in this report: Metformin (PubChem CID: 4091); Fasudil (PubChem CID: 3547); Palmitic acid (PubChem CID: 985). |
| first_indexed | 2024-12-11T11:43:06Z |
| format | Article |
| id | doaj.art-5179173b03f54408a1244bc34d3b7564 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T11:43:06Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-5179173b03f54408a1244bc34d3b75642022-12-22T01:08:33ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-07-011010.3389/fphar.2019.00811435482Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent PathwayCuihua Jiang0Cuihua Jiang1Cuihua Jiang2Yiting Wang3Qiaomei Jin4Qiaomei Jin5Dongjian Zhang6Dongjian Zhang7Meng Gao8Meng Gao9Nan Yao10Nan Yao11Zhiqi Yin12Jian Zhang13Jian Zhang14Shiping Ma15Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, ChinaThis study aimed to assess the effects of triterpene extract of Cyclocarya paliurus (Batal.) Iljinskaja (CPT) on diabetes-induced hepatic inflammation and to unveil the underlying mechanisms. Diabetes in db/db mice was alleviated after CPT administration, as assessed by the oral glucose tolerance test. In addition, treatment with CPT dramatically reduced serum insulin, aspartate amino-transaminase, alanine aminotransferase, triglyceride, and total cholesterol amounts. Besides, serum levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were also reduced after CPT administration. Western blot analysis revealed that CPT treatment significantly reversed the protein expression levels of Rho, ROCK1, ROCK2, p-P65, p-IκBα, p-IKKα, and p-IKKβ in liver samples obtained from db/db mice. Upon palmitic acid stimulation, the protective effects of CPT on the liver were further assessed in HepG2 and LO2 cells, and no appreciable cytotoxic effects were found. Therefore, these findings indicate that CPT alleviates liver inflammation via Rho-kinase signaling.Chemical compounds evaluated in this report: Metformin (PubChem CID: 4091); Fasudil (PubChem CID: 3547); Palmitic acid (PubChem CID: 985).https://www.frontiersin.org/article/10.3389/fphar.2019.00811/fullCyclocarya paliurustriterpenediabetes mellitushepatic inflammationROCKNF-κB |
| spellingShingle | Cuihua Jiang Cuihua Jiang Cuihua Jiang Yiting Wang Qiaomei Jin Qiaomei Jin Dongjian Zhang Dongjian Zhang Meng Gao Meng Gao Nan Yao Nan Yao Zhiqi Yin Jian Zhang Jian Zhang Shiping Ma Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway Frontiers in Pharmacology Cyclocarya paliurus triterpene diabetes mellitus hepatic inflammation ROCK NF-κB |
| title | Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway |
| title_full | Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway |
| title_fullStr | Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway |
| title_full_unstemmed | Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway |
| title_short | Cyclocarya paliurus Triterpenoids Improve Diabetes-Induced Hepatic Inflammation via the Rho-Kinase-Dependent Pathway |
| title_sort | cyclocarya paliurus triterpenoids improve diabetes induced hepatic inflammation via the rho kinase dependent pathway |
| topic | Cyclocarya paliurus triterpene diabetes mellitus hepatic inflammation ROCK NF-κB |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00811/full |
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