Usiigaci: Instance-aware cell tracking in stain-free phase contrast microscopy enabled by machine learning

Stain-free, single-cell segmentation and tracking is tantamount to the holy grail of microscopic cell migration analysis. Phase contrast microscopy (PCM) images with cells at high density are notoriously difficult to segment accurately; thus, manual segmentation remains the de facto standard practic...

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Bibliographic Details
Main Authors: Hsieh-Fu Tsai, Joanna Gajda, Tyler F.W. Sloan, Andrei Rares, Amy Q. Shen
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:SoftwareX
Online Access:http://www.sciencedirect.com/science/article/pii/S2352711018301882