Usiigaci: Instance-aware cell tracking in stain-free phase contrast microscopy enabled by machine learning
Stain-free, single-cell segmentation and tracking is tantamount to the holy grail of microscopic cell migration analysis. Phase contrast microscopy (PCM) images with cells at high density are notoriously difficult to segment accurately; thus, manual segmentation remains the de facto standard practic...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2019-01-01
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Series: | SoftwareX |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2352711018301882 |