Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli

Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli

The insufficient supply of sugar units is the key limitation for the biosynthesis of glycosylated products. The unusual sugar TDP-l-mycarose is initially attached to the C3 of the polyketide erythronolide B, resulting in 3-O-α-mycarosylerythronolide B (MEB). Here, we present the de novo biosynthesis...

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Bibliographic Details
Main Authors: Zhifeng Liu, Jianlin Xu, Zhanguang Feng, Yong Wang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-06-01
Series:Synthetic and Systems Biotechnology
Subjects:
TDP-l-mycarose
Glycosylated products
3-O-α-mycarosylerythronolide B
Metabolic engineering
CRISPRi
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X22000266

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http://www.sciencedirect.com/science/article/pii/S2405805X22000266

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