Real-time monitoring of recombinant GFP expression in single-cell Komagataella phaffii through filamentous pelletization technology

Real-time monitoring of recombinant GFP expression in single-cell Komagataella phaffii through filamentous pelletization technology

Komagataella phaffii is an important cell factory for recombinant protein production under methanol induction. Tracking of recombinant protein expression by single K. phaffii cell is a hot topic to identify the variation of expression level in submerge cultivation system. In this study, a platform s...

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Bibliographic Details
Main Authors: Zheng Xiao, Cong Wenjie, Zhou Hualan, Zhang Jianguo
Format: Article
Language:English
Published: De Gruyter 2023-10-01
Series:Reviews in Analytical Chemistry
Subjects:
on-line single cell analysis
cell immobilization
fungal pellets
komagataella phaffii
Online Access:https://doi.org/10.1515/revac-2023-0059

Internet

https://doi.org/10.1515/revac-2023-0059

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