Using PrISMa to reveal the interactome of the human claudins family

Summary: Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them...

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Bibliographic Details
Main Authors: Daniel Perez-Hernandez, Lorena Suarez-Artiles, Mattson S.O. Jones, Gunnar Dittmar
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005166