Differential in vivo labeling with barcoded antibodies allows for simultaneous transcriptomic profiling of airway, lung tissue and intravascular immune cells

Differential in vivo labeling with barcoded antibodies allows for simultaneous transcriptomic profiling of airway, lung tissue and intravascular immune cells

Single-cell RNA sequencing (scRNA-seq) is the state-of-the-art approach to study transcriptomic signatures in individual cells in respiratory health and disease. However, classical scRNA-seq approaches provide no spatial information and are performed using either bronchoalveolar lavage fluid (BAL) o...

Full description

Bibliographic Details
Main Authors: Barbara C. Mindt, John Kim, Troy Warren, Yang Song, Antonio DiGiandomenico
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Immunology
Subjects:
respiratory disease
in vivo antibody labeling
spatial transcriptomics
scRNA-seq
bacterial lung infection
neutrophils
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227175/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227175/full

Similar Items