IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction

IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction

Abstract Background Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amounts of high-quality genomic DNA. Other PCR-based techniques are either i...

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Bibliographic Details
Main Authors: Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn, Hoo Sun Chung, Dirk Inzé, Stephen Depuydt
Format: Article
Language:English
Published: BMC 2022-12-01
Series:Plant Methods
Subjects:
Copy number determination
Competitive PCR
T-DNA insertions
IMPLANT
Genetically modified plants
Online Access:https://doi.org/10.1186/s13007-022-00965-0

Internet

https://doi.org/10.1186/s13007-022-00965-0

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