Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery
High-grade serous epithelial ovarian carcinoma (HGSOC) is an immunogenic tumor with a unique tumor microenvironment (TME) that extends to the peritoneal cavity. The immunosuppressive nature of TME imposes the major challenge to develop effective treatment options for HGSOC. Interaction of immune cel...
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MDPI AG
2022-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/12/2121 |
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| author | Pavan Kumar Samruddhi Ranmale Hemant Tongaonkar Jayanti Mania-Pramanik |
| author_facet | Pavan Kumar Samruddhi Ranmale Hemant Tongaonkar Jayanti Mania-Pramanik |
| author_sort | Pavan Kumar |
| collection | DOAJ |
| description | High-grade serous epithelial ovarian carcinoma (HGSOC) is an immunogenic tumor with a unique tumor microenvironment (TME) that extends to the peritoneal cavity. The immunosuppressive nature of TME imposes the major challenge to develop effective treatment options for HGSOC. Interaction of immune cells in TME is an important factor. Hence, a better understanding of immune profile of TME may be required for exploring alternative treatment options. Immune profiling of peritoneal fluid (PF), tumor specimens, and blood were carried out using flowcytometry, ELISA, and Procartaplex immunoassay. The frequency of CD56<sup>Bright</sup>NK cells and expression of functional receptors were reduced in PF. Increased activating NKp46+CD56<sup>Dim</sup>NK cells may indicate differential antitumor response in PF. Functional receptors on NK, NKT-like and T cells were reduced more drastically in tumor specimens. Soluble ligands MIC-B and PVR were reduced, whereas B7-H6 was increased in PF. Dissemination of tumor cells contributes to soluble ligands in PF. A differential cytokine profile was found in serum and PF as IL-2, IL-8, IL-15, IL-27, IFN-γ, and GM-CSF were elevated specifically in PF. In conclusion, the differential immune profile and correlation of soluble parameters and NK cell receptors with chemo response score may add knowledge to understand anti-tumor immune response to develop effective treatment modality. |
| first_indexed | 2024-03-09T15:45:41Z |
| format | Article |
| id | doaj.art-51dea0a4df05450ea1e9125303d430a5 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T15:45:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-51dea0a4df05450ea1e9125303d430a52023-11-24T18:33:08ZengMDPI AGVaccines2076-393X2022-12-011012212110.3390/vaccines10122121Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking SurgeryPavan Kumar0Samruddhi Ranmale1Hemant Tongaonkar2Jayanti Mania-Pramanik3ICMR-National Institute for Research in Reproductive and Child Health, Mumbai 400012, Maharashtra, IndiaICMR-National Institute for Research in Reproductive and Child Health, Mumbai 400012, Maharashtra, IndiaP. D. Hinduja National Hospital & Medical Research Centre, Mumbai 400016, Maharashtra, IndiaICMR-National Institute for Research in Reproductive and Child Health, Mumbai 400012, Maharashtra, IndiaHigh-grade serous epithelial ovarian carcinoma (HGSOC) is an immunogenic tumor with a unique tumor microenvironment (TME) that extends to the peritoneal cavity. The immunosuppressive nature of TME imposes the major challenge to develop effective treatment options for HGSOC. Interaction of immune cells in TME is an important factor. Hence, a better understanding of immune profile of TME may be required for exploring alternative treatment options. Immune profiling of peritoneal fluid (PF), tumor specimens, and blood were carried out using flowcytometry, ELISA, and Procartaplex immunoassay. The frequency of CD56<sup>Bright</sup>NK cells and expression of functional receptors were reduced in PF. Increased activating NKp46+CD56<sup>Dim</sup>NK cells may indicate differential antitumor response in PF. Functional receptors on NK, NKT-like and T cells were reduced more drastically in tumor specimens. Soluble ligands MIC-B and PVR were reduced, whereas B7-H6 was increased in PF. Dissemination of tumor cells contributes to soluble ligands in PF. A differential cytokine profile was found in serum and PF as IL-2, IL-8, IL-15, IL-27, IFN-γ, and GM-CSF were elevated specifically in PF. In conclusion, the differential immune profile and correlation of soluble parameters and NK cell receptors with chemo response score may add knowledge to understand anti-tumor immune response to develop effective treatment modality.https://www.mdpi.com/2076-393X/10/12/2121ovarian cancertumor microenvironmentNK cell receptorsimmune modulationcognate ligands |
| spellingShingle | Pavan Kumar Samruddhi Ranmale Hemant Tongaonkar Jayanti Mania-Pramanik Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery Vaccines ovarian cancer tumor microenvironment NK cell receptors immune modulation cognate ligands |
| title | Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery |
| title_full | Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery |
| title_fullStr | Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery |
| title_full_unstemmed | Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery |
| title_short | Immune Profile of Blood, Tissue and Peritoneal Fluid: A Comparative Study in High Grade Serous Epithelial Ovarian Cancer Patients at Interval Debulking Surgery |
| title_sort | immune profile of blood tissue and peritoneal fluid a comparative study in high grade serous epithelial ovarian cancer patients at interval debulking surgery |
| topic | ovarian cancer tumor microenvironment NK cell receptors immune modulation cognate ligands |
| url | https://www.mdpi.com/2076-393X/10/12/2121 |
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