Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices
Summary: Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, ta...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005385 |