Elimination of bla KPC−2-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system
Abstract Objective The purpose of this study is to re-sensitive bacteria to carbapenemases and reduce the transmission of the bla KPC−2 gene by curing the bla KPC−2-harboring plasmid of carbapenem-resistant using the CRISPR-Cas9 system. Methods The single guide RNA (sgRNA) specifically targeted to t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-10-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-03058-7 |