Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing

Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing

Specifically ablating genes in human induced pluripotent stem cells (iPSCs) allows for studies of gene function as well as disease mechanisms in disorders caused by loss-of-function (LOF) mutations. While techniques exist for engineering such lines, we have developed and rigorously validated a metho...

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Bibliographic Details
Main Authors: Andrew M. Tidball, Louis T. Dang, Trevor W. Glenn, Emma G. Kilbane, Daniel J. Klarr, Joshua L. Margolis, Michael D. Uhler, Jack M. Parent
Format: Article
Language:English
Published: Elsevier 2017-09-01
Series:Stem Cell Reports
Subjects:
CRISPR/Cas9
gene editing
induced pluripotent stem cells
reprogramming
genetic epilepsy
epileptic encephalopathy
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117303132

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http://www.sciencedirect.com/science/article/pii/S2213671117303132

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