Utilizing Dichloromethane as an Extremely Proficient Substitute for Phenol/Chloroform in Extracting RNA with Exceptional Purity from Woody Tissues of Coconut

Procuring high-grade RNA from mature coconut tissues is a tricky and labor-intensive process due to the intricate scaffold of polysaccharides, polyphenols, lipids, and proteins that form firm complexes with nucleic acids. However, we have effectively developed a novel method for the first time, lett...

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Bibliographic Details
Main Authors: Amjad Iqbal, Yaodong Yang
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/6/5/75