Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes

Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes

After synthesis of transmembrane proteins (TMPs), they are transferred and inserted into plasma membranes to play biological functions. Crucially, orientation of TMPs in membranes determines whether they have biological activities. In cellular environments, a number of cofactors, such as translocon,...

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Bibliographic Details
Main Authors: Haihong Huang, Baosheng Ge, Shuai Zhang, Jiqiang Li, Chenghao Sun, Tongtao Yue, Fang Huang
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Materials
Subjects:
transmembrane protein
mimic membrane
insertion
orientation
fluorescence quenching titration
Online Access:https://www.mdpi.com/1996-1944/12/3/349

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https://www.mdpi.com/1996-1944/12/3/349

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