Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage
Objective: The study aimed to explore the antioxidant activity and stability of Lateolabrax maculatus protein hydrolysates (LPH) in vitro. Methods: Chemical experiments were used to determine the antioxidant activity of LPH and analyze the effects of pH, temperature, metal ions (K+, Ca2+, Cu2+, Zn2+...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-02-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050194 |
| _version_ | 1811169220526866432 |
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| author | Qun GAO Yuannan HUANG Xixi CAI Yixiao WANG Ming DU Yongle LIU Shaoyun WANG |
| author_facet | Qun GAO Yuannan HUANG Xixi CAI Yixiao WANG Ming DU Yongle LIU Shaoyun WANG |
| author_sort | Qun GAO |
| collection | DOAJ |
| description | Objective: The study aimed to explore the antioxidant activity and stability of Lateolabrax maculatus protein hydrolysates (LPH) in vitro. Methods: Chemical experiments were used to determine the antioxidant activity of LPH and analyze the effects of pH, temperature, metal ions (K+, Ca2+, Cu2+, Zn2+) and simulated gastrointestinal digestion on its stability. And the oxidative damage model of Caco-2 cells induced by hydrogen peroxide was used to explore the protective effect of LPH against cellular oxidative damage. Results: LPH had strong free radical scavenging activity against DPPH and ABTS and the IC50 values were 2.13 mg/mL and 31.53 μg/mL respectively. LPH was stable to pH(2.0~12.0), temperature (25~100 ℃) and K+ (0.25~2 mmol/L). Ca2+ and Cu2+ at 0.25~2 mmol/L improved DPPH scavenging rate of LPH, while Zn2+ above 1 mmol/L decreased it. In addition, LPH had good gastrointestinal stability. In the Caco-2 cell model induced by hydrogen peroxide, LPH significantly increased cell viability from 58.02% to 83.40% (P<0.05), significantly inhibited the release of intracellular reactive oxygen species (P<0.05) and restored mitochondrial membrane potential to normal level. Furthermore, LPH significantly inhibited the activity of lactate dehydrogenase in supernatant to 19.34% of model group (P<0.05) and significantly increased the activities of superoxide dismutase and catalase in cells (P<0.05). Conclusion: LPH had good antioxidant activity and stability, suggesting a good application prospect in the area of functional nutritious food. |
| first_indexed | 2024-04-10T16:38:49Z |
| format | Article |
| id | doaj.art-529e4ce1de954494ae2b759858b85860 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T16:38:49Z |
| publishDate | 2023-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-529e4ce1de954494ae2b759858b858602023-02-08T08:00:16ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-02-0144441041810.13386/j.issn1002-0306.20220501942022050194-4Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative DamageQun GAO0Yuannan HUANG1Xixi CAI2Yixiao WANG3Ming DU4Yongle LIU5Shaoyun WANG6College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha 410114, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaObjective: The study aimed to explore the antioxidant activity and stability of Lateolabrax maculatus protein hydrolysates (LPH) in vitro. Methods: Chemical experiments were used to determine the antioxidant activity of LPH and analyze the effects of pH, temperature, metal ions (K+, Ca2+, Cu2+, Zn2+) and simulated gastrointestinal digestion on its stability. And the oxidative damage model of Caco-2 cells induced by hydrogen peroxide was used to explore the protective effect of LPH against cellular oxidative damage. Results: LPH had strong free radical scavenging activity against DPPH and ABTS and the IC50 values were 2.13 mg/mL and 31.53 μg/mL respectively. LPH was stable to pH(2.0~12.0), temperature (25~100 ℃) and K+ (0.25~2 mmol/L). Ca2+ and Cu2+ at 0.25~2 mmol/L improved DPPH scavenging rate of LPH, while Zn2+ above 1 mmol/L decreased it. In addition, LPH had good gastrointestinal stability. In the Caco-2 cell model induced by hydrogen peroxide, LPH significantly increased cell viability from 58.02% to 83.40% (P<0.05), significantly inhibited the release of intracellular reactive oxygen species (P<0.05) and restored mitochondrial membrane potential to normal level. Furthermore, LPH significantly inhibited the activity of lactate dehydrogenase in supernatant to 19.34% of model group (P<0.05) and significantly increased the activities of superoxide dismutase and catalase in cells (P<0.05). Conclusion: LPH had good antioxidant activity and stability, suggesting a good application prospect in the area of functional nutritious food.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050194sea bassantioxidant peptidesstabilityhydrogen peroxidecaco-2 cells |
| spellingShingle | Qun GAO Yuannan HUANG Xixi CAI Yixiao WANG Ming DU Yongle LIU Shaoyun WANG Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage Shipin gongye ke-ji sea bass antioxidant peptides stability hydrogen peroxide caco-2 cells |
| title | Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage |
| title_full | Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage |
| title_fullStr | Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage |
| title_full_unstemmed | Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage |
| title_short | Stability Analysis of Antioxidant Peptides from Lateolabrax maculatus and Their Protective Effect Against Cellular Oxidative Damage |
| title_sort | stability analysis of antioxidant peptides from lateolabrax maculatus and their protective effect against cellular oxidative damage |
| topic | sea bass antioxidant peptides stability hydrogen peroxide caco-2 cells |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050194 |
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