Structure- and Content-Dependent Efficiency of Cas9-Assisted DNA Cleavage in Genome-Editing Systems

Structure- and Content-Dependent Efficiency of Cas9-Assisted DNA Cleavage in Genome-Editing Systems

Genome-editing systems, being some of the key tools of molecular biologists, represent a reasonable hope for progress in the field of personalized medicine. A major problem with such systems is their nonideal accuracy and insufficient selectivity. The selectivity of CRISPR-Cas9 systems can be improv...

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Bibliographic Details
Main Authors: Svetlana V. Baranova, Polina V. Zhdanova, Alexander A. Lomzov, Vladimir V. Koval, Alexander A. Chernonosov
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
CRISPR-Cas system
Cas9 activity
oligonucleotide mismatch
cleavage
thermodynamics
Online Access:https://www.mdpi.com/1422-0067/23/22/13889

Internet

https://www.mdpi.com/1422-0067/23/22/13889

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