TILLING-by-Sequencing<sup>+</sup> to Decipher Oil Biosynthesis Pathway in Soybeans: A New and Effective Platform for High-Throughput Gene Functional Analysis

TILLING-by-Sequencing<sup>+</sup> to Decipher Oil Biosynthesis Pathway in Soybeans: A New and Effective Platform for High-Throughput Gene Functional Analysis

Reverse genetic approaches have been widely applied to study gene function in crop species; however, these techniques, including gel-based TILLING, present low efficiency to characterize genes in soybeans due to genome complexity, gene duplication, and the presence of multiple gene family members th...

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Bibliographic Details
Main Authors: Naoufal Lakhssassi, Zhou Zhou, Mallory A. Cullen, Oussama Badad, Abdelhalim El Baze, Oumaima Chetto, Mohamed G. Embaby, Dounya Knizia, Shiming Liu, Leandro G. Neves, Khalid Meksem
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:International Journal of Molecular Sciences
Subjects:
TILLING by target capture sequencing
TILLING-by-sequencing<sup>+</sup>
oil biosynthesis pathway
fatty acid desaturases
<i>GmSACPD-C</i>
<i>GmFAD2-1A/1B</i>
Online Access:https://www.mdpi.com/1422-0067/22/8/4219

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https://www.mdpi.com/1422-0067/22/8/4219

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