A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality

Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties. Such modified molecules are in high demand for basic research and therapeutic applications such as cancer treatment and...

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Bibliographic Details
Main Authors: Simon K. Krebs, Nathanaël Rakotoarinoro, Marlitt Stech, Anne Zemella, Stefan Kubick
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.873906/full