A ClearSee-Based Clearing Protocol for 3D Visualization of <i>Arabidopsis thaliana</i> Embryos

A ClearSee-Based Clearing Protocol for 3D Visualization of <i>Arabidopsis thaliana</i> Embryos

Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable clearing method that is applicable to a wide range of tissues and is suitable for gene expression analysis using fluorescent reporters, but its applic...

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Bibliographic Details
Main Authors: Ayame Imoto, Mizuki Yamada, Takumi Sakamoto, Airi Okuyama, Takashi Ishida, Shinichiro Sawa, Mitsuhiro Aida
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Plants
Subjects:
clearing
3D imaging
<i>Arabidopsis thaliana</i>
embryo
confocal microscopy
cell wall staining
Online Access:https://www.mdpi.com/2223-7747/10/2/190

Internet

https://www.mdpi.com/2223-7747/10/2/190

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