MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
Background: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression o...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023089831 |
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| author | Elham Khalili Ali Afgar Azam Rajabpour Seyed Hamid Aghaee-Bakhtiari Khadijeh Jamialahmadi Ladan Teimoori-Toolabi |
| author_facet | Elham Khalili Ali Afgar Azam Rajabpour Seyed Hamid Aghaee-Bakhtiari Khadijeh Jamialahmadi Ladan Teimoori-Toolabi |
| author_sort | Elham Khalili |
| collection | DOAJ |
| description | Background: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression of Tyms and Abcg2 as resistance-inducing genes in the resistant cell lines to 5-Fluorouracil. Methods: 5-Fluorouracil-resistant HCT116 and SW480 cell lines were generated by consecutive treatment of cells with 5-Fluorouracil. This resistance induction was validated by MTT assays. The expression of the Tyms and Abcg2 gene and miR-548c-3p were studied by quantitative real-time PCR in the cell lines. Results: We hypothesized that miR-548c-3p is targeting Tyms and Abcg2 simultaneously. Increased expression Tyms gene in the two most resistant cell lines derived from HCT116 and all resistant cell lines derived from SW480 except one were seen. Increased expression of Abcg2 was observed in the most resistant HCT116-derived cell line and all resistant cell lines, derived from SW480. In all resistant cell lines, the expression of miR-548c-3p was decreased. Conclusion: It can be concluded downregulation of miR548c-3p is in line with Tyms and Abcg2 overexpression in resistant cell lines to 5-Fluorouracil. |
| first_indexed | 2024-03-09T09:17:42Z |
| format | Article |
| id | doaj.art-52ce70e8f52a47a1822533010ad7aa01 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:17:42Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-52ce70e8f52a47a1822533010ad7aa012023-12-02T07:04:12ZengElsevierHeliyon2405-84402023-11-01911e21775MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracilElham Khalili0Ali Afgar1Azam Rajabpour2Seyed Hamid Aghaee-Bakhtiari3Khadijeh Jamialahmadi4Ladan Teimoori-Toolabi5Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, IranResearch Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, IranMolecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, IranDepartment of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Corresponding author. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Iran; Corresponding author. Molecular Medicine Department, Pasteur Institute of Iran, Tehran, IranBackground: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression of Tyms and Abcg2 as resistance-inducing genes in the resistant cell lines to 5-Fluorouracil. Methods: 5-Fluorouracil-resistant HCT116 and SW480 cell lines were generated by consecutive treatment of cells with 5-Fluorouracil. This resistance induction was validated by MTT assays. The expression of the Tyms and Abcg2 gene and miR-548c-3p were studied by quantitative real-time PCR in the cell lines. Results: We hypothesized that miR-548c-3p is targeting Tyms and Abcg2 simultaneously. Increased expression Tyms gene in the two most resistant cell lines derived from HCT116 and all resistant cell lines derived from SW480 except one were seen. Increased expression of Abcg2 was observed in the most resistant HCT116-derived cell line and all resistant cell lines, derived from SW480. In all resistant cell lines, the expression of miR-548c-3p was decreased. Conclusion: It can be concluded downregulation of miR548c-3p is in line with Tyms and Abcg2 overexpression in resistant cell lines to 5-Fluorouracil.http://www.sciencedirect.com/science/article/pii/S2405844023089831Colorectal neoplasmDrug resistance5-FluorouracilTYMS proteinHumanATP binding cassette transporter |
| spellingShingle | Elham Khalili Ali Afgar Azam Rajabpour Seyed Hamid Aghaee-Bakhtiari Khadijeh Jamialahmadi Ladan Teimoori-Toolabi MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil Heliyon Colorectal neoplasm Drug resistance 5-Fluorouracil TYMS protein Human ATP binding cassette transporter |
| title | MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil |
| title_full | MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil |
| title_fullStr | MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil |
| title_full_unstemmed | MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil |
| title_short | MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil |
| title_sort | mir 548c 3p through suppressing tyms and abcg2 increases the sensitivity of colorectal cancer cells to 5 fluorouracil |
| topic | Colorectal neoplasm Drug resistance 5-Fluorouracil TYMS protein Human ATP binding cassette transporter |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023089831 |
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