Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer
One of the disadvantages of gel preparations is the poor ability to penetrate the skin. Herein we developed a sodium carboxymethylcellulose (NaCMC)-based gel containing microparticle of doxycycline hyclate (DOX). Oleic acid (OA) was added into the gel formulation specifically to increase the penetr...
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| Format: | Article |
| Language: | English |
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Universitas Ahmad Dahlan
2021-07-01
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| Series: | Pharmaciana |
| Subjects: | |
| Online Access: | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/21044/pdf_195 |
| _version_ | 1818404737230307328 |
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| author | Aliyah Aliyah Windy Winalda Oktaviana Kadek Saka Dwipayanti Arini Putri Erdiana Rifka Nurul Utami Andi Dian Permana |
| author_facet | Aliyah Aliyah Windy Winalda Oktaviana Kadek Saka Dwipayanti Arini Putri Erdiana Rifka Nurul Utami Andi Dian Permana |
| author_sort | Aliyah Aliyah |
| collection | DOAJ |
| description | One of the disadvantages of gel preparations is the poor ability to penetrate the skin. Herein we developed a sodium carboxymethylcellulose (NaCMC)-based gel containing microparticle of doxycycline hyclate (DOX). Oleic acid (OA) was added into the gel formulation specifically to increase the penetrability of DOX microparticles. The objective of this work was to determine the effect of varying concentration of OA on the physical characteristics, penetration and retention abilities of DOX. DOX microparticles were initially prepared using PLGA as a matrix and then incorporated into a NaCMC-based gel with various OA concentrations: 0%, 2.5%, 5%, 7.5%, and 10% for F1, F2, F3, F4, and F5, respectively. The gel preparations were evaluated for their organoleptic test, homogeneity, pH measurement, viscosity, spreadability, as well as ex vivo penetration and retention abilities. The physical characteristics tests revealed a homogeneous yellow gel with a distinctive odor and pH values compatible with the pH requirements of human skin. The retention test showed the formulation retained 1236.46 µg of DOX in the skin. Finally, the skin retention of DOX from microparticles loaded gel was significantly higher compared to the free DOX loaded gel, indicating the microparticles can be extremely effective in retaining the DOX in the infected area. |
| first_indexed | 2024-12-14T08:44:54Z |
| format | Article |
| id | doaj.art-52d757a5447c4432abedfabedb7c05e8 |
| institution | Directory Open Access Journal |
| issn | 2088-4559 2477-0256 |
| language | English |
| last_indexed | 2024-12-14T08:44:54Z |
| publishDate | 2021-07-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj.art-52d757a5447c4432abedfabedb7c05e82022-12-21T23:09:11ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562021-07-0111223925010.12928/pharmaciana.v11i2.21044Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancerAliyah Aliyah0Windy Winalda Oktaviana1Kadek Saka Dwipayanti2Arini Putri Erdiana3Rifka Nurul Utami4 Andi Dian Permana5Department of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaOne of the disadvantages of gel preparations is the poor ability to penetrate the skin. Herein we developed a sodium carboxymethylcellulose (NaCMC)-based gel containing microparticle of doxycycline hyclate (DOX). Oleic acid (OA) was added into the gel formulation specifically to increase the penetrability of DOX microparticles. The objective of this work was to determine the effect of varying concentration of OA on the physical characteristics, penetration and retention abilities of DOX. DOX microparticles were initially prepared using PLGA as a matrix and then incorporated into a NaCMC-based gel with various OA concentrations: 0%, 2.5%, 5%, 7.5%, and 10% for F1, F2, F3, F4, and F5, respectively. The gel preparations were evaluated for their organoleptic test, homogeneity, pH measurement, viscosity, spreadability, as well as ex vivo penetration and retention abilities. The physical characteristics tests revealed a homogeneous yellow gel with a distinctive odor and pH values compatible with the pH requirements of human skin. The retention test showed the formulation retained 1236.46 µg of DOX in the skin. Finally, the skin retention of DOX from microparticles loaded gel was significantly higher compared to the free DOX loaded gel, indicating the microparticles can be extremely effective in retaining the DOX in the infected area.http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/21044/pdf_195oleic acidgeldoxycycline microparticlepenetration enhancer |
| spellingShingle | Aliyah Aliyah Windy Winalda Oktaviana Kadek Saka Dwipayanti Arini Putri Erdiana Rifka Nurul Utami Andi Dian Permana Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer Pharmaciana oleic acid gel doxycycline microparticle penetration enhancer |
| title | Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer |
| title_full | Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer |
| title_fullStr | Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer |
| title_full_unstemmed | Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer |
| title_short | Enhanced skin localization of doxycycline using microparticles and hydrogel: effect of oleic acid as penetration enhancer |
| title_sort | enhanced skin localization of doxycycline using microparticles and hydrogel effect of oleic acid as penetration enhancer |
| topic | oleic acid gel doxycycline microparticle penetration enhancer |
| url | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/21044/pdf_195 |
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