A simple method to make, trap and deform a vesicle in a gel

Abstract We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characte...

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Bibliographic Details
Main Authors: Pierre Tapie, Alexis M. Prevost, Lorraine Montel, Léa-Laetitia Pontani, Elie Wandersman
Format: Article
Language:English
Published: Nature Portfolio 2023-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-31996-9