Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

Summary: High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a pro...

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Bibliographic Details
Main Authors: Ian H. Guldner, Samantha M. Golomb, Qingfei Wang, Emilia Wang, Siyuan Zhang
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002446