Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

Macromolecular complexes that exhibit continuous forms of structural flexibility pose a challenge for many existing tools in cryo-EM single-particle analysis. We describe a new tool, called multi-body refinement, which models flexible complexes as a user-defined number of rigid bodies that move inde...

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Bibliographic Details
Main Authors: Takanori Nakane, Dari Kimanius, Erik Lindahl, Sjors HW Scheres
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-06-01
Series:eLife
Subjects:
cryo-electron microscopy
single-particle analysis
maximum likelihood
image processing
focused refinement
Online Access:https://elifesciences.org/articles/36861

Internet

https://elifesciences.org/articles/36861

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