Efficient deletion of microRNAs using CRISPR/Cas9 with dual guide RNAs

Efficient deletion of microRNAs using CRISPR/Cas9 with dual guide RNAs

MicroRNAs (miRNAs) are short non-coding RNAs that play crucial roles in gene regulation, exerting post-transcriptional silencing, thereby influencing cellular function, development, and disease. Traditional loss-of-function methods for studying miRNA functions, such as miRNA inhibitors and sponges,...

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Main Authors: Smitha Ijee, Karthik Chambayil, Anurag Dutta Chaudhury, Abhirup Bagchi, Kirti Modak, Saswati Das, Esther Sathya Bama Benjamin, Sonam Rani, Daniel Zechariah Paul, Aneesha Nath, Debanjan Roy, Dhavapriya Palani, Sweety Priyanka, Rakshini Ravichandran, Betty K. Kumary, Yazhini Sivamani, Vijayanand S., Dinesh Babu, Yukio Nakamura, Vasanth Thamodaran, Poonkuzhali Balasubramanian, Shaji R. Velayudhan
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-04-01
Series:Frontiers in Molecular Biosciences
Subjects:
miRNA
CRISPR-Cas9
dual-gRNA
iPSCs
erythroid
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2023.1295507/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2023.1295507/full

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