Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i>
Group A <i>Streptococcus</i> (GAS) causes a variety of diseases globally. The DNases in GAS promote GAS evasion of neutrophil killing by degrading neutrophil extracellular traps (NETs). Sda1 is a prophage-encoded DNase associated with virulent GAS strains. However, protective immunity ag...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/1/102 |
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| author | Shuai Bi Jie Wang Meiyi Xu Ning Li Beinan Wang |
| author_facet | Shuai Bi Jie Wang Meiyi Xu Ning Li Beinan Wang |
| author_sort | Shuai Bi |
| collection | DOAJ |
| description | Group A <i>Streptococcus</i> (GAS) causes a variety of diseases globally. The DNases in GAS promote GAS evasion of neutrophil killing by degrading neutrophil extracellular traps (NETs). Sda1 is a prophage-encoded DNase associated with virulent GAS strains. However, protective immunity against Sda1 has not been determined. In this study, we explored the potential of Sda1 as a vaccine candidate. Sda1 was used as a vaccine to immunize mice intranasally. The effect of anti-Sda1 IgG in neutralizing degradation of NETs was determined and the protective role of Sda1 was investigated with intranasal and systemic challenge models. Antigen-specific antibodies were induced in the sera and pharyngeal mucosal site after Sda1 immunization. The anti-Sda1 IgG efficiently prevented degradation of NETs by supernatant samples from different GAS serotypes with or without Sda1. Sda1 immunization promoted clearance of GAS from the nasopharynx independent of GAS serotypes but did not reduce lethality after systemic GAS challenge. Anti-Sda1 antibody can neutralize degradation of NETs by Sda1 and other phage-encoded DNases and decrease GAS colonization at the nasopharynx across serotypes. These results indicate that Sda1 can be a potential vaccine candidate for reduction in GAS reservoir and GAS tonsillitis-associated diseases. |
| first_indexed | 2024-03-10T00:22:42Z |
| format | Article |
| id | doaj.art-534f3703f48e4e49af1f0a11be4c46ca |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T00:22:42Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-534f3703f48e4e49af1f0a11be4c46ca2023-11-23T15:39:28ZengMDPI AGVaccines2076-393X2022-01-0110110210.3390/vaccines10010102Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i>Shuai Bi0Jie Wang1Meiyi Xu2Ning Li3Beinan Wang4Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaGroup A <i>Streptococcus</i> (GAS) causes a variety of diseases globally. The DNases in GAS promote GAS evasion of neutrophil killing by degrading neutrophil extracellular traps (NETs). Sda1 is a prophage-encoded DNase associated with virulent GAS strains. However, protective immunity against Sda1 has not been determined. In this study, we explored the potential of Sda1 as a vaccine candidate. Sda1 was used as a vaccine to immunize mice intranasally. The effect of anti-Sda1 IgG in neutralizing degradation of NETs was determined and the protective role of Sda1 was investigated with intranasal and systemic challenge models. Antigen-specific antibodies were induced in the sera and pharyngeal mucosal site after Sda1 immunization. The anti-Sda1 IgG efficiently prevented degradation of NETs by supernatant samples from different GAS serotypes with or without Sda1. Sda1 immunization promoted clearance of GAS from the nasopharynx independent of GAS serotypes but did not reduce lethality after systemic GAS challenge. Anti-Sda1 antibody can neutralize degradation of NETs by Sda1 and other phage-encoded DNases and decrease GAS colonization at the nasopharynx across serotypes. These results indicate that Sda1 can be a potential vaccine candidate for reduction in GAS reservoir and GAS tonsillitis-associated diseases.https://www.mdpi.com/2076-393X/10/1/102group A <i>streptococcus</i>DNasesSda1neutrophil extracellular trapspharyngeal colonization |
| spellingShingle | Shuai Bi Jie Wang Meiyi Xu Ning Li Beinan Wang Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> Vaccines group A <i>streptococcus</i> DNases Sda1 neutrophil extracellular traps pharyngeal colonization |
| title | Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> |
| title_full | Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> |
| title_fullStr | Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> |
| title_full_unstemmed | Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> |
| title_short | Immunity to Sda1 Protects against Infection by Sda1<sup>+</sup> and Sda1<sup>−</sup> Serotypes of Group A <i>Streptococcus</i> |
| title_sort | immunity to sda1 protects against infection by sda1 sup sup and sda1 sup sup serotypes of group a i streptococcus i |
| topic | group A <i>streptococcus</i> DNases Sda1 neutrophil extracellular traps pharyngeal colonization |
| url | https://www.mdpi.com/2076-393X/10/1/102 |
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