Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization.

Optimized protocols for achieving high-yield expression, purification and reconstitution of membrane proteins are required to study their structure and function. We previously reported high-level expression in Escherichia coli of active BmrC and BmrD proteins from Bacillus subtilis, previously named...

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Bibliographic Details
Main Authors: Carmen Galián, Florence Manon, Manuela Dezi, Cristina Torres, Christine Ebel, Daniel Lévy, Jean-Michel Jault
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3094339?pdf=render