Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine
Food allergy is a type I allergic reaction induced by mast cells and is mainly activated by allergen-specific immunoglobulin (Ig)E. Spi-B is an E26-transformation-specific (Ets) family transcription factor essential for the differentiation and functional maturation of several immune cell subsets, in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Allergy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/falgy.2022.996657/full |
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| author | Narumi Ishihara Yutaka Nakamura Yutaka Nakamura Kyosuke Yakabe Seiga Komiyama Yumiko Fujimura Tsuneyasu Kaisho Tsuneyasu Kaisho Shunsuke Kimura Shunsuke Kimura Koji Hase Koji Hase Koji Hase |
| author_facet | Narumi Ishihara Yutaka Nakamura Yutaka Nakamura Kyosuke Yakabe Seiga Komiyama Yumiko Fujimura Tsuneyasu Kaisho Tsuneyasu Kaisho Shunsuke Kimura Shunsuke Kimura Koji Hase Koji Hase Koji Hase |
| author_sort | Narumi Ishihara |
| collection | DOAJ |
| description | Food allergy is a type I allergic reaction induced by mast cells and is mainly activated by allergen-specific immunoglobulin (Ig)E. Spi-B is an E26-transformation-specific (Ets) family transcription factor essential for the differentiation and functional maturation of several immune cell subsets, including mast cells. However, the possible involvement of Spi-B in food allergy remains unclear. In this study, we found that Spi-B-deficient mice were highly susceptible to food allergy to ovalbumin (OVA), as indicated by the exacerbation of diarrhea and elevation of serum IgE levels. These pathological changes were associated with enhanced mast cell infiltration into the intestinal lamina propria. Activation of mast cells in the intestinal mucosa was observed in Spib−/− mice, even under physiological conditions. Accordingly, Spi-B deficiency increased the translocation of fluorescently labeled dextran from the lumen to the serum, suggesting increased intestinal permeability in Spib−/− mice. Moreover, Spib−/− mice showed defects in oral tolerance induction to OVA. These data illustrate that Spi-B suppresses the development of food allergies by controlling the activation of intestinal mast cells and by inducing immune tolerance to food allergens. |
| first_indexed | 2024-04-11T19:05:09Z |
| format | Article |
| id | doaj.art-53b2f1b39ea94fc8a85cc97a3437b6fd |
| institution | Directory Open Access Journal |
| issn | 2673-6101 |
| language | English |
| last_indexed | 2024-04-11T19:05:09Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Allergy |
| spelling | doaj.art-53b2f1b39ea94fc8a85cc97a3437b6fd2022-12-22T04:07:48ZengFrontiers Media S.A.Frontiers in Allergy2673-61012022-10-01310.3389/falgy.2022.996657996657Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestineNarumi Ishihara0Yutaka Nakamura1Yutaka Nakamura2Kyosuke Yakabe3Seiga Komiyama4Yumiko Fujimura5Tsuneyasu Kaisho6Tsuneyasu Kaisho7Shunsuke Kimura8Shunsuke Kimura9Koji Hase10Koji Hase11Koji Hase12Division of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanDepartment of Microbiology and Immunology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanDepartment of Immunology, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, JapanLaboratory for Inflammatory Regulation, RIKEN Center for Integrative Medical Science (IMS-RCAI), Yokohama, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanPrecursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Saitama, JapanDivision of Biochemistry, Faculty of Pharmacy, School of Pharmaceutical Sciences, Keio University, Tokyo, JapanInstitute of Fermentation Sciences (IFeS), Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, JapanInternational Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo (IMSUT), Tokyo, JapanFood allergy is a type I allergic reaction induced by mast cells and is mainly activated by allergen-specific immunoglobulin (Ig)E. Spi-B is an E26-transformation-specific (Ets) family transcription factor essential for the differentiation and functional maturation of several immune cell subsets, including mast cells. However, the possible involvement of Spi-B in food allergy remains unclear. In this study, we found that Spi-B-deficient mice were highly susceptible to food allergy to ovalbumin (OVA), as indicated by the exacerbation of diarrhea and elevation of serum IgE levels. These pathological changes were associated with enhanced mast cell infiltration into the intestinal lamina propria. Activation of mast cells in the intestinal mucosa was observed in Spib−/− mice, even under physiological conditions. Accordingly, Spi-B deficiency increased the translocation of fluorescently labeled dextran from the lumen to the serum, suggesting increased intestinal permeability in Spib−/− mice. Moreover, Spib−/− mice showed defects in oral tolerance induction to OVA. These data illustrate that Spi-B suppresses the development of food allergies by controlling the activation of intestinal mast cells and by inducing immune tolerance to food allergens.https://www.frontiersin.org/articles/10.3389/falgy.2022.996657/fullSpi-Bfood allergyepithelial barrierIgEoral tolerance |
| spellingShingle | Narumi Ishihara Yutaka Nakamura Yutaka Nakamura Kyosuke Yakabe Seiga Komiyama Yumiko Fujimura Tsuneyasu Kaisho Tsuneyasu Kaisho Shunsuke Kimura Shunsuke Kimura Koji Hase Koji Hase Koji Hase Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine Frontiers in Allergy Spi-B food allergy epithelial barrier IgE oral tolerance |
| title | Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| title_full | Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| title_fullStr | Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| title_full_unstemmed | Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| title_short | Spi-B alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| title_sort | spi b alleviates food allergy by securing mucosal barrier and immune tolerance in the intestine |
| topic | Spi-B food allergy epithelial barrier IgE oral tolerance |
| url | https://www.frontiersin.org/articles/10.3389/falgy.2022.996657/full |
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