Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses
Abstract Background Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to mouse small intestine to create 2D mouse intestinal epithelial monolayer...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-08-01
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| Series: | BMC Cell Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12860-018-0165-0 |