Method for CRISPR/Cas9 Mutagenesis in Candida albicans

Candida albicans is the most prevalent and important human fungal pathogen. The advent of CRISPR as a means of gene editing has greatly facilitated genetic analysis in C. albicans. Here, we describe a detailed step-by-step procedure to construct and analyze C. albicans deletion mutants. This protoco...

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Bibliographic Details
Main Authors: Neta Dean, Henry Ng
Format: Article
Language:English
Published: Bio-protocol LLC 2018-04-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2814&type=0