A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transm...

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Bibliographic Details
Main Authors: Réza Shahidi, Elizabeth A Williams, Markus Conzelmann, Albina Asadulina, Csaba Verasztó, Sanja Jasek, Luis A Bezares-Calderón, Gáspár Jékely
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2015-12-01
Series:eLife
Subjects:
Platynereis dumerilii
annelid
neuropeptide
connectome
immunogold
Online Access:https://elifesciences.org/articles/11147

Internet

https://elifesciences.org/articles/11147

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