ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Abstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RN...

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Bibliographic Details
Main Authors: Helena García-Castro, Nathan J. Kenny, Marta Iglesias, Patricia Álvarez-Campos, Vincent Mason, Anamaria Elek, Anna Schönauer, Victoria A. Sleight, Jakke Neiro, Aziz Aboobaker, Jon Permanyer, Manuel Irimia, Arnau Sebé-Pedrós, Jordi Solana
Format: Article
Language:English
Published: BMC 2021-04-01
Series:Genome Biology
Subjects:
Single-cell transcriptomics
Planarian
RNA-seq
Fixation
Dissociation
SPLiT-seq
Online Access:https://doi.org/10.1186/s13059-021-02302-5

Internet

https://doi.org/10.1186/s13059-021-02302-5

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