ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
Abstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RN...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-04-01
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| Series: | Genome Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13059-021-02302-5 |
| _version_ | 1818456218431127552 |
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| author | Helena García-Castro Nathan J. Kenny Marta Iglesias Patricia Álvarez-Campos Vincent Mason Anamaria Elek Anna Schönauer Victoria A. Sleight Jakke Neiro Aziz Aboobaker Jon Permanyer Manuel Irimia Arnau Sebé-Pedrós Jordi Solana |
| author_facet | Helena García-Castro Nathan J. Kenny Marta Iglesias Patricia Álvarez-Campos Vincent Mason Anamaria Elek Anna Schönauer Victoria A. Sleight Jakke Neiro Aziz Aboobaker Jon Permanyer Manuel Irimia Arnau Sebé-Pedrós Jordi Solana |
| author_sort | Helena García-Castro |
| collection | DOAJ |
| description | Abstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life. |
| first_indexed | 2024-12-14T22:23:11Z |
| format | Article |
| id | doaj.art-545233f391c049b29663104de0e9910a |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-12-14T22:23:11Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-545233f391c049b29663104de0e9910a2022-12-21T22:45:27ZengBMCGenome Biology1474-760X2021-04-0122113410.1186/s13059-021-02302-5ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomicsHelena García-Castro0Nathan J. Kenny1Marta Iglesias2Patricia Álvarez-Campos3Vincent Mason4Anamaria Elek5Anna Schönauer6Victoria A. Sleight7Jakke Neiro8Aziz Aboobaker9Jon Permanyer10Manuel Irimia11Arnau Sebé-Pedrós12Jordi Solana13Department of Biological and Medical Sciences, Oxford Brookes UniversityDepartment of Biological and Medical Sciences, Oxford Brookes UniversityCentre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST)Department of Biological and Medical Sciences, Oxford Brookes UniversityDepartment of Biological and Medical Sciences, Oxford Brookes UniversityCentre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST)Department of Biological and Medical Sciences, Oxford Brookes UniversitySchool of Biological Sciences, University of AberdeenDepartment of Zoology, University of OxfordDepartment of Zoology, University of OxfordCentre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST)Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST)Department of Biological and Medical Sciences, Oxford Brookes UniversityAbstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.https://doi.org/10.1186/s13059-021-02302-5Single-cell transcriptomicsPlanarianRNA-seqFixationDissociationSPLiT-seq |
| spellingShingle | Helena García-Castro Nathan J. Kenny Marta Iglesias Patricia Álvarez-Campos Vincent Mason Anamaria Elek Anna Schönauer Victoria A. Sleight Jakke Neiro Aziz Aboobaker Jon Permanyer Manuel Irimia Arnau Sebé-Pedrós Jordi Solana ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics Genome Biology Single-cell transcriptomics Planarian RNA-seq Fixation Dissociation SPLiT-seq |
| title | ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| title_full | ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| title_fullStr | ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| title_full_unstemmed | ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| title_short | ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| title_sort | acme dissociation a versatile cell fixation dissociation method for single cell transcriptomics |
| topic | Single-cell transcriptomics Planarian RNA-seq Fixation Dissociation SPLiT-seq |
| url | https://doi.org/10.1186/s13059-021-02302-5 |
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