A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets

A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets

Summary: Transcriptional changes happen within minutes; however, RNAi or genetic deletion requires days to weeks before transcription networks can be analyzed. This limitation has made it challenging to distinguish direct from indirect targets of sequence-specific transcription factors. This inabili...

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Bibliographic Details
Main Authors: Hillary M. Layden, Nicholas A. Eleuteri, Scott W. Hiebert, Kristy R. Stengel
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Flow Cytometry/Mass Cytometry
Genomics
Molecular Biology
CRISPR
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002379

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http://www.sciencedirect.com/science/article/pii/S2666166721002379

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