Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer
The prostate specific membrane antigen (PSMA), extensively overexpressed on prostate cancer (PCa) cell surface, has been validated as a diagnostic biomarker for PCa. However, insufficient attention has been paid to the development of PSMA-specific probes loaded with small chemical molecules for the...
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MDPI AG
2022-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/10/2051 |
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| author | Wei Zhou Jiandong Huang Qingwei Xiao Shunmin Hu Shijia Li Jie Zheng Zhiyun Du Jiangling Peng Huixiong Chen |
| author_facet | Wei Zhou Jiandong Huang Qingwei Xiao Shunmin Hu Shijia Li Jie Zheng Zhiyun Du Jiangling Peng Huixiong Chen |
| author_sort | Wei Zhou |
| collection | DOAJ |
| description | The prostate specific membrane antigen (PSMA), extensively overexpressed on prostate cancer (PCa) cell surface, has been validated as a diagnostic biomarker for PCa. However, insufficient attention has been paid to the development of PSMA-specific probes loaded with small chemical molecules for the in vivo molecular imaging of PCa. In this study, we innovatively labelled superparamagnetic iron oxide nanoparticles with a PSMA-targeting Glu-Urea-Lys scaffold. An optimized synthetic route was developed to offer a physiochemically stable probe. The probe demonstrated high binding affinity (0.38 ± 0.08 μg(Fe)/mL) and binding specificity to PSMA expressed on prostate cancer cell surface in vitro. In a xenograft PCa mouse model, significant negative contrast of the implanted prostate cancer xenograft could be specifically observed by MRI 6 h after tail vein injection of the tracer (Fe, 20 mg/kg), exhibiting its potential to exclusively enhance magnetic resonance detection of PCa. |
| first_indexed | 2024-03-09T19:35:30Z |
| format | Article |
| id | doaj.art-547e953dcb814b4b99342e108444dd16 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T19:35:30Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-547e953dcb814b4b99342e108444dd162023-11-24T01:54:45ZengMDPI AGPharmaceutics1999-49232022-09-011410205110.3390/pharmaceutics14102051Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate CancerWei Zhou0Jiandong Huang1Qingwei Xiao2Shunmin Hu3Shijia Li4Jie Zheng5Zhiyun Du6Jiangling Peng7Huixiong Chen8School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaSchool of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, 100 Waihuanxi Road, Guangzhou 510006, ChinaThe prostate specific membrane antigen (PSMA), extensively overexpressed on prostate cancer (PCa) cell surface, has been validated as a diagnostic biomarker for PCa. However, insufficient attention has been paid to the development of PSMA-specific probes loaded with small chemical molecules for the in vivo molecular imaging of PCa. In this study, we innovatively labelled superparamagnetic iron oxide nanoparticles with a PSMA-targeting Glu-Urea-Lys scaffold. An optimized synthetic route was developed to offer a physiochemically stable probe. The probe demonstrated high binding affinity (0.38 ± 0.08 μg(Fe)/mL) and binding specificity to PSMA expressed on prostate cancer cell surface in vitro. In a xenograft PCa mouse model, significant negative contrast of the implanted prostate cancer xenograft could be specifically observed by MRI 6 h after tail vein injection of the tracer (Fe, 20 mg/kg), exhibiting its potential to exclusively enhance magnetic resonance detection of PCa.https://www.mdpi.com/1999-4923/14/10/2051prostate cancerPSMAmolecular imagingMRI contrast agentSPIONs |
| spellingShingle | Wei Zhou Jiandong Huang Qingwei Xiao Shunmin Hu Shijia Li Jie Zheng Zhiyun Du Jiangling Peng Huixiong Chen Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer Pharmaceutics prostate cancer PSMA molecular imaging MRI contrast agent SPIONs |
| title | Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer |
| title_full | Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer |
| title_fullStr | Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer |
| title_full_unstemmed | Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer |
| title_short | Glu-Urea-Lys Scaffold Functionalized Superparamagnetic Iron Oxide Nanoparticles Targeting PSMA for In Vivo Molecular MRI of Prostate Cancer |
| title_sort | glu urea lys scaffold functionalized superparamagnetic iron oxide nanoparticles targeting psma for in vivo molecular mri of prostate cancer |
| topic | prostate cancer PSMA molecular imaging MRI contrast agent SPIONs |
| url | https://www.mdpi.com/1999-4923/14/10/2051 |
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