Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly
Abstract Background The spaceflight environment is an extreme environment that affects the immune system of approximately 50% of astronauts. With planned long-duration missions, such as the deployment of the Lunar Gateway and possible interplanetary missions, it is mandatory to determine how all com...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Immunity & Ageing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12979-023-00393-1 |
| _version_ | 1797451470766866432 |
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| author | Coralie Fonte Pauline Jacob Anne Vanet Stéphanie Ghislin Jean-Pol Frippiat |
| author_facet | Coralie Fonte Pauline Jacob Anne Vanet Stéphanie Ghislin Jean-Pol Frippiat |
| author_sort | Coralie Fonte |
| collection | DOAJ |
| description | Abstract Background The spaceflight environment is an extreme environment that affects the immune system of approximately 50% of astronauts. With planned long-duration missions, such as the deployment of the Lunar Gateway and possible interplanetary missions, it is mandatory to determine how all components of the immune system are affected, which will allow the establishment of countermeasures to preserve astronaut health. However, despite being an important component of the immune system, antibody-mediated humoral immunity has rarely been investigated in the context of the effects of the space environment. It has previously been demonstrated that 30 days aboard the BION-M1 satellite and 21 days of hindlimb unloading (HU), a model classically used to mimic the effects of microgravity, decrease murine B lymphopoiesis. Furthermore, modifications in B lymphopoiesis reported in young mice subjected to 21 days of HU were shown to be similar to those observed in aged mice (18–22 months). Since the primary antibody repertoire composed of IgM is created by V(D) J recombination during B lymphopoiesis, the objective of this study was to assess the degree of similarity between changes in the bone marrow IgM repertoire and in the V(D)J recombination process in 2.5-month-old mice subjected to 21 days of HU and aged (18 months) mice. Results We found that in 21 days, HU induced changes in the IgM repertoire that were approximately 3-fold less than those in aged mice, which is a rapid effect. Bone remodeling and epigenetics likely mediate these changes. Indeed, we previously demonstrated a significant decrease in tibial morphometric parameters from day 6 of HU and a progressive reduction in these parameters until day 21 of HU, and it has been shown that age and microgravity induce epigenetic changes. Conclusion These data reveal novel immune changes that are akin to advanced aging and underline the importance of studying the effects of spaceflight on antibody-mediated humoral immunity. |
| first_indexed | 2024-03-09T14:55:08Z |
| format | Article |
| id | doaj.art-5487ef889dc74769913ee3cad7c1e2d4 |
| institution | Directory Open Access Journal |
| issn | 1742-4933 |
| language | English |
| last_indexed | 2024-03-09T14:55:08Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Immunity & Ageing |
| spelling | doaj.art-5487ef889dc74769913ee3cad7c1e2d42023-11-26T14:13:58ZengBMCImmunity & Ageing1742-49332023-11-0120111710.1186/s12979-023-00393-1Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less stronglyCoralie Fonte0Pauline Jacob1Anne Vanet2Stéphanie Ghislin3Jean-Pol Frippiat4Stress Immunity Pathogens Laboratory, UR 7300 SIMPA, Faculty of Medicine, Lorraine UniversityStress Immunity Pathogens Laboratory, UR 7300 SIMPA, Faculty of Medicine, Lorraine UniversityUniversité Paris Cité, CNRS, Institut Jacques MonodStress Immunity Pathogens Laboratory, UR 7300 SIMPA, Faculty of Medicine, Lorraine UniversityStress Immunity Pathogens Laboratory, UR 7300 SIMPA, Faculty of Medicine, Lorraine UniversityAbstract Background The spaceflight environment is an extreme environment that affects the immune system of approximately 50% of astronauts. With planned long-duration missions, such as the deployment of the Lunar Gateway and possible interplanetary missions, it is mandatory to determine how all components of the immune system are affected, which will allow the establishment of countermeasures to preserve astronaut health. However, despite being an important component of the immune system, antibody-mediated humoral immunity has rarely been investigated in the context of the effects of the space environment. It has previously been demonstrated that 30 days aboard the BION-M1 satellite and 21 days of hindlimb unloading (HU), a model classically used to mimic the effects of microgravity, decrease murine B lymphopoiesis. Furthermore, modifications in B lymphopoiesis reported in young mice subjected to 21 days of HU were shown to be similar to those observed in aged mice (18–22 months). Since the primary antibody repertoire composed of IgM is created by V(D) J recombination during B lymphopoiesis, the objective of this study was to assess the degree of similarity between changes in the bone marrow IgM repertoire and in the V(D)J recombination process in 2.5-month-old mice subjected to 21 days of HU and aged (18 months) mice. Results We found that in 21 days, HU induced changes in the IgM repertoire that were approximately 3-fold less than those in aged mice, which is a rapid effect. Bone remodeling and epigenetics likely mediate these changes. Indeed, we previously demonstrated a significant decrease in tibial morphometric parameters from day 6 of HU and a progressive reduction in these parameters until day 21 of HU, and it has been shown that age and microgravity induce epigenetic changes. Conclusion These data reveal novel immune changes that are akin to advanced aging and underline the importance of studying the effects of spaceflight on antibody-mediated humoral immunity.https://doi.org/10.1186/s12979-023-00393-1AntibodySpaceflightHumoral immunityStressImmune systemB lymphopoiesis |
| spellingShingle | Coralie Fonte Pauline Jacob Anne Vanet Stéphanie Ghislin Jean-Pol Frippiat Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly Immunity & Ageing Antibody Spaceflight Humoral immunity Stress Immune system B lymphopoiesis |
| title | Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly |
| title_full | Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly |
| title_fullStr | Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly |
| title_full_unstemmed | Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly |
| title_short | Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly |
| title_sort | hindlimb unloading a physiological model of microgravity modifies the murine bone marrow igm repertoire in a similar manner as aging but less strongly |
| topic | Antibody Spaceflight Humoral immunity Stress Immune system B lymphopoiesis |
| url | https://doi.org/10.1186/s12979-023-00393-1 |
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