Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

ABSTRACT Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in sample multiplexing is becoming necessary...

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Bibliographic Details
Main Authors: Johanna B. Holm, Michael S. Humphrys, Courtney K. Robinson, Matthew L. Settles, Sandra Ott, Li Fu, Hongqiu Yang, Pawel Gajer, Xin He, Elias McComb, Patti E. Gravitt, Khalil G. Ghanem, Rebecca M. Brotman, Jacques Ravel
Format: Article
Language:English
Published: American Society for Microbiology 2019-02-01
Series:mSystems
Subjects:
16S RNA
amplicon sequencing
PCR
ultrahigh throughput
Online Access:https://journals.asm.org/doi/10.1128/msystems.00029-19

Internet

https://journals.asm.org/doi/10.1128/msystems.00029-19

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